Seed Extract Attenuates Glycolipid Metabolism Disorder in Hyperlipidemia Mice Through PPAR Signaling Pathway Based on Metabolomics and Network Pharmacology.

Hyperlipidemia is characterized by a high level of blood lipid which poses a serious threat to human health. seed is a traditional crop of medicine and food homology with a wide range of pharmacological actions. To make clear the attenuation effect of seed against hyperlipidemia, low and high doses of seed extract (CSE) were orally administered to hyperlipidemia model mice developed by high-fat diet (HFD).

Hotspot Analysis of Rumen Microbiota and Methane Mitigation in Ruminants: A Bibliometric Analysis from 1998 to 2023.

Methane (CH) is the second-most abundant greenhouse gas, following carbon dioxide (CO), and has a warming potential 28 times greater than CO [...

Solvent-free processing of lignin into robust room temperature phosphorescent materials.

Producing room temperature phosphorescent (RTP) materials from biomass resources using a solvent free method is essential but hard to achieve. Here, we discovered that lignin dissolved well in the liquid monomer, 2-hydroxyethyl acrylate (HEA), due to extensive hydrogen bonding and non-bonding interactions between lignin and HEA. Motivated by this discovery, we developed a solvent free system consisting of HEA and urethane dimethacrylate (UDMA) for converting lignin into RTP materials.

Atractylenolide I Attenuates Glucocorticoid-Induced Osteoporosis via Inhibiting NF-κB Signaling Pathway.

Long-term treatment with glucocorticoids significantly impacts bone health, with glucocorticoid-induced osteoporosis (GIOP) being the most prevalent consequence. Previous studies have established that Atractylenolide I (Atr I) possesses anti-inflammatory, antioxidant and anti-tumor properties, however, its specific effects on osteoclastogenesis and GIOP are still unclear. In this study, our in vitro results revealed that Atr I inhibited RANKL-stimulated osteoclast differentiation in a dose-dependent manner, disrupted the structure of the F-actin belt in mature osteoclasts, blocked RANKL-induced ROS production, and suppressed the expression of osteoclast-associated genes.

The emergence of highly resistant and hypervirulent Escherichia coli ST405 clone in a tertiary hospital over 8 years.

The emergence of carbapenem-resistant (CREC) poses crucial challenges in clinical management, requiring continuous monitoring to inform control and treatment strategies. This study aimed to investigate the genomic and epidemiological characteristics of CREC isolates obtained from a tertiary hospital in China between 2015 and 2022. Next-generation sequencing was used for genomic profiling, and clinical data from patients were integrated into the analysis.

The application of tissue engineering strategies for uterine regeneration.

Uterine injuries, particularly damages to endometrium, are usually associated with abnormal menstruation, recurrent miscarriage, pregnancy complications, and infertility. Tissue engineering using cell-based, biomolecule-based, or biomaterial and scaffold-based strategies has emerged as a novel and promising approach for uterine regeneration. Stem cells, biomolecules, and porous scaffolds used alone or, very often, used in combination as a more effective treatment means have shown great potential in promoting uterine regeneration.

Genome-wide characterization and expression profiling of the gene family in sweet orange () reveal responses to multiple phytohormones and abiotic stresses.

Citrus is widely recognized as one of the most economically important fruit crops worldwide. However, citrus growth is frequently hindered by external environmental stresses, which severely limit its development and yield. The TGA (TGACG motif-binding factor) transcription factors (TFs) are members of the bZIP family and play essential roles in plant defense responses and organ development.

Superiority of Ga-PSMA-11 PET/CT over mpMRI for lateralization accuracy of diagnosing intra-glandular prostate cancer lesions: avoiding fluke targeting.

Objective: The aim of this study was to compare the diagnostic accuracy of Ga-PSMA-11 PET/CT and multiparametric MRI (mpMRI) in detecting unilateral and bilateral intra-glandular prostate cancer lesions.

Methods: A retrospective analysis was conducted on 73 prostate cancer patients diagnosed via biopsy, all of whom underwent both Ga-PSMA-11 PET/CT and mpMRI prior to surgery. Two independent readers, blinded to each other's results and to pathology findings, evaluated the imaging modalities to make a diagnosis of unilateral (left or right) or bilateral lesions for suspected prostate lesions.

DCE-MRI quantitative analysis and MRI-based radiomics for predicting the early efficacy of microwave ablation in lung cancers.

Objectives: To evaluate the feasibility and value of dynamic contrast-enhanced MRI (DCE-MRI) quantitative analysis and MRI-based radiomics in predicting the efficacy of microwave ablation (MWA) in lung cancers (LCs).

Methods: Forty-three patients with LCs who underwent DCE-MRI within 24 h of receiving MWA were enrolled in the study and divided into two groups according to the modified response evaluation criteria in solid tumors (m-RECIST) criteria: the effective treatment (complete response + partial response + stable disease, n = 28) and the ineffective treatment (progressive disease, n = 15). DCE-MRI datasets were processed by Omni.

Subjective Cognitive Decline in COPD - A Cross-Sectional Analysis of the Behavioral Risk Factor Surveillance System.

Background: Subjective cognitive decline (SCD), an early indicator of cognitive impairment, may affect COPD care and outcomes, yet its sociodemographic associations in Chronic Obstructive Pulmonary Disease (COPD) remain poorly understood.

Objective: To investigate the prevalence of SCD among people with COPD across different age groups and identify associations between demographics, risk behaviors, comorbidities, and self-reported SCD.

Design: Pooled 2019 BRFSS data from 24 states using the Cognitive Decline module were analyzed.

Collaborative filtering based on GNN with attribute fusion and broad attention.

Recommender systems based on collaborative filtering (CF) have been a prominent area of research. In recent years, graph neural networks (GNN) based CF models have effectively addressed the limitations of nonlinearity and higher-order feature interactions in traditional recommendation methods, such as matrix decomposition-based methods and factorization machine approaches, achieving excellent recommendation performance. However, existing GNN-based CF models still have two problems that affect performance improvement.

Unveiling the Hidden Mechanism: How LuxS/AI-2 quorum sensing system drives antimicrobial resistance in Salmonella pullorum through activation of the efflux pump AcrAB-TolC.

Understanding the antimicrobial resistance (AMR) mechanism of Salmonella pullorum (SP), which is widespread among yellow chickens in China, is crucial for reducing significant economic losses in the industry. In this study, we explored the AMR mechanism by which the LuxS/AI-2-mediated quorum sensing (QS) system regulates the AcrAB-TolC efflux pump in the SP. The results showed that the luxS gene and the signaling molecule AI-2 had no effect on the growth of the SP strains.

Piperlongumine alleviates viral myocarditis by inhibiting pyroptosis through NF-κB pathway.

Background: Viral myocarditis (VMC) is a cardiac condition characterized by inflammation of the myocardium due to viral infection. It is a significant cause of sudden cardiac death in young adults, and effective treatments remain limited. Inflammatory caspase-mediated pyroptosis serves as a host defense mechanism against pathogens and is crucial in the pathogenesis of VMC.

Microbial dysbiosis with tryptophan metabolites alteration in lower respiratory tract is associated with clinical responses to anti-PD-1 immunotherapy in advanced non-small cell lung cancer.

Lower respiratory tract microbiome constitutes a unique immune microenvironment for advanced non-small cell lung cancer as one of dominant localized microbial components. However, there exists little knowledge on the associations between this regional microbiome and clinical responses to anti-PD-1 immunotherapy from clinical perspectives. Here, we equivalently collected bronchoalveolar lavage fluids from 56 advanced NSCLC participants treated with none (untreated, n = 28) or anti-PD-1 immunotherapy (treated, n = 28), which was further divided into responder (n = 17) and non-responder (n = 11) subgroups according to clinical responses, aiming to compare their microbial discrepancy by performing metagenomic sequencing and targeted metabolic alterations by tryptophan sequencing.

The association between uric acid to high-density cholesterol ratio and depression: A population-based cross-sectional study.

Background: Depression is associated with inflammation, and the uric acid to HDL-C cholesterol ratio (UHR) has emerged as a potential marker of increased inflammation; however, the association between UHR and depression is unclear. Therefore, we aimed to explore this association in a sample from the general US population.

Methods: We conducted a cross-sectional study of 11,444 participants ≥20 years of age from the 2009-2014 NHANES database.

Habitat-Dependent DNA viral communities in atmospheric aerosols: Insights from terrestrial and marine ecosystems in East Asia.

The transmission of viruses through aerosols is of growing public health concern, yet research on aerosol-associated viral communities lags behind that of terrestrial and aquatic ecosystems. Here, DNA viral diversity in natural aerosols from both over land and ocean in the East Asia region was examined. The results showed that atmospheric environments harbor a distinct viral community that differs from those present in terrestrial and aquatic ecosystems.

Combined genomic, transcriptomic, and metabolomic analyses provide insights into the fruit development of bottle gourd ().

Bottle gourd ( (Molina) Standl) is a widely distributed Cucurbitaceae species, but gaps and low-quality assemblies have limited its genomic study. To address this, we assembled a nearly complete, high-quality genome of the bottle gourd (Pugua) using PacBio HiFi sequencing and Hi-C correction. The genome, being 298.

Outbreaks of Ulva prolifera green tides reduce the network complexity and stability of cooccurring planktonic microbial communities.

Ulva prolifera green tides are becoming a worldwide environmental problem, especially in the Yellow Sea, China. However, the effects of the occurrence of U. prolifera green tides on the community organization and stability of surrounding microbiomes have still not been determined.

Mechanistic insights into EIF6 as a target of Apigenin in alleviating chondrocyte senescence.

Purpose: Apigenin, a flavonoid found in edible plants, has demonstrated therapeutic potential in various diseases, but its role in knee osteoarthritis (KOA) remains unclear. This study aimed to identify the potential targets and mechanisms of Apigenin in KOA.

Methods: Network pharmacology analysis identified 80 targets of Apigenin, of which 48 overlapped with KOA-related targets.

Schwann cells secrete IGFBP5 to facilitate the growth of keloids.

Keloids (KD) are noncancerous fibroproliferative tumors exhibiting cancer-like traits, encompass aggressive unregulated growth, absence of natural regression, and a significantly high rate of recurrence. The precise molecular mechanisms underlying KD pathology remain poorly understood. In this study, we employed single-cell sequencing to examine the characteristics of cells in KD and normal scar (NS) tissue.

Unveiling Ion-Pairing and Anion Dual Charge Carrier in Ca-Organic Batteries.

Calcium-organic batteries offer sustainable energy storage with high voltage, yet their reaction mechanisms remain unclear. Here, a hidden dual-ion charge storage mechanism is unveiled in poly(anthraquinone imide) (PAQI) using Ca(TFSI)₂/ether-based electrolytes, challenging the conventional Ca⁺-only storage model. It is demonstrated that Ca⁺-TFSI⁻ ion pairs co-bind to carbonyl groups during the first electron transfer, followed by TFSI⁻ dissociation to activate adjacent carbonyl sites in the second step-a process reversible upon charging.

VITAP: a high precision tool for DNA and RNA viral classification based on meta-omic data.

The rapid growth in the number of newly identified DNA and RNA viral sequences underscores the need for an accurate and comprehensive classification system for all viral realms at different taxonomic levels. Here, we establish the Viral Taxonomic Assignment Pipeline (VITAP), which addresses classification challenges by integrating alignment-based techniques with graphs, offering high precision in classifying both DNA and RNA viral sequences and providing confidence level for each taxonomic unit. This tool automatically updates its database in sync with the latest references from the International Committee on Taxonomy of Viruses (ICTV), efficiently classifying viral sequences as short as 1,000 base pairs to genus level.

Peripheral immune profiling highlights a dynamic role of low-density granulocytes in myasthenia gravis.

Background: Myasthenia gravis (MG) is an autoimmune neuromuscular disease marked by dysregulation of several immune cell populations. Here we explored peripheral immune landscape, particularly the role of low-density granulocytes (LDGs).

Methods: Single-cell and bulk RNA sequencing analyzed peripheral immune cells from MG patients pre- (n = 4) and after treatment (n = 2), as well as healthy controls (n = 3).

Paternal fenvalerate exposure causes depressive-like behaviour by altering Grb10 gene DNA methylation in adolescent offspring.

Fenvalerate, a typical pyrethroid pesticide, is a neurological toxicant. This study aimed to evaluate the effect of paternal exposure to fenvalerate on depressive-like behaviours in adolescent offspring. Depression-like behavior was determined by Sucrose Preference Test (SPT), Tail Suspension Test (TST) and Forced Swimming Test (FST) in adolescent offspring.

New insights into the transcription factor regulatory networks driving peel coloration under hormone induction analyzed by transcriptomics and metabolomics in tangor 'Murcot'.

Introduction: Fruit color is a crucial quality factor strongly influencing consumer preference for citrus. The coloration of citrus fruit is primarily determined by carotenoids, which produce a range of hues. Gibberellic acid (GA) and ethylene are critical in fruit coloration during the ripening process.