The potential of exosomes from adipose-derived stromal-vascular fraction in Increasing Migration Activity of Human Dental Pulp Stromal Cells (in vitro ).

Background: Migration of dental pulp stromal cells (DPSCs) significantly responds to wound healing after pulp injury. Deriving from low compliance characteristics, pulp tissue regeneration is challenging and depends on the microenvironmental signals. Exosomes can maintain and carry bioactive proteins that are crucial in cell communication.

Potential Use of L-Arginine Amino Acids towards Proliferation and Migratory Speed Rate of Human Dental Pulp Stem Cells.

Objective: L-arginine is a semi-essential amino acid produced by the body which has an important role in the process of stem cell regeneration. However, under inflammatory conditions, denaturation of pulp amino acids and proteins occurr resulting in a decrease in the ability of stem cells to self-renew. Therefore, in this study, L-arginine was added in vitro to the culture media Dulbecco's Modified Eagle Medium - (DMEM) of human dental pulp stem cells (hDPSCs) to analyse the potential of L-arginine on migration and proliferation by comparing between 3 concentrations, namely 300, 400, 500 μmol/L and control group (DMEM), to obtain the most optimal concentration for proliferation and migration.

Iodine Density Threshold to Distinguish Between Enhancing and Nonenhancing Renal Lesions With Dual-Layer Dual-Energy CT.

Purpose: This study aimed to determine the optimal threshold iodine density to distinguish enhancing and nonenhancing renal masses with dual-layer dual-energy CT (dlDECT).

Methods: In this retrospective, HIPAA-compliant, institutional review board-approved study, 383 consecutive renal mass dlDECT studies from September 5, 2018, through December 15, 2022, were reviewed for enhancing solid renal masses with ≥∆20 HU. Studies with simple cysts in the same interval served as controls.

The Effect of Hydrogel Hyaluronic Acid on Dentine Sialophosphoprotein Expression of Human Dental Pulp Stem Cells.

Objective: Hyaluronic acid (HA) is glycosaminoglycan and one of important factors in extracellular matrix. In an inflamed pulp, when niche biology is conducive, the recruitment of human dental pulp stem cells (hDPSCs) will take place and differentiate into odontoblast like cell, creating reparative dentine and expressing dentine sialophosphoprotein (DSPP). Therefore, the purpose of this study was to analyze the potential of hydrogel HA in various concentration towards hDPSCs differentiation via DSPP expression at day 7 and 14.

Viability of human dental pulp stem cells: The potential of L-arginine-based culture media.

Dental pulp is built by proteins that have various roles in the biological process of pulp, such as structural protein, regulation protein, and catalytic protein. L-arginine, an amino acid and one of the building blocks of proteins, regulates pro-inflammatory and anti-inflammatory activity. Therefore, L-arginine-based culture has potential to promote dental pulp regeneration.

Application of non-degradable waste as building material for low-cost housing.

Building material is one of the essential aspects in accommodating the supply and demand of low-cost housing in Indonesia. Recently, several researchers have devoted much time and effort to developing waste recycling for building materials since it is more ecologically benign, particularly for non-degradable waste. This article focuses on recycling disposable diaper waste as composite material for a structural and architectural component of the building based on Indonesian building standards.

Characterization, Physical Properties, and Biocompatibility of Novel Tricalcium Silicate-Chitosan Endodontic Sealer.

Objective:  The purpose of this study was to compare the characteristics, physical properties, and biocompatibility of the novel tricalcium silicate-chitosan (TCS-C) sealer with AH Plus and Sure-Seal Root.

Materials And Methods:  The TCS-C powder was prepared by mixing tricalcium silicate with 2% water-soluble chitosan at a 5:1 ratio, followed by sufficient addition of 10 g/mL ratio of double-distilled water to form a homogeneous cement. Material characterizations (the Fourier Transform InfraRed [FTIR] and X-ray diffraction [XRD]), physical property investigations (flow and film thickness), and cytotoxicity tests in 3T3 mouse embryo fibroblast cell (MTT assay method) were performed on sealers, and the results were compared with those of the commercial products.

Effect of etch-and-rinse and self-etch modes in universal adhesives on push-out bond strength of fiber post.

Background: Nowadays, the universal adhesive become more popular among clinicians due to its simple procedure. The application of universal adhesive on root canal dentin prior the self-adhesive resin cement may increase bond-strength between fiber post and dentin. The objective of this study was to evaluate the effect of different etching modes (etch-and-rinse and self-etch) to universal adhesives on push-out bond strength of fiber post.

Holistic care of patients with diabetic foot ulcers during the COVID-19 era: integration of Henderson's Need Theory.

The COVID-19 pandemic has inhibited the practice of diabetic foot ulcer care, particularly in the community. Comprehensive theory-based nursing care is needed to prevent further complications. Unfortunately, a study combining theory with nursing care in diabetic foot ulcer care has not been explored.

Analysis of Thrombin-Activated Platelet-Derived Exosome (T-aPDE) Potential for Dental Pulp Regeneration: In-Vitro Study.

Objective:  This study analyzed the potential of various concentrations of the thrombin-activated platelet-derived exosome (T-aPDE) to regenerate the dental pulp by performing an analysis of the cell viability, migration activity, and vascular endothelial growth factor A (VEGF-A) expression of human dental pulp stem cells (hDPSCs).

Material And Methods:  The hDPSCs were collected from nine third molar teeth of nine healthy donors and were isolated and cultured using the explant method. They were harvested between the third and fourth passages and starved, after which they were seeded in the following treatments: Dulbecco's Modified Eagle Medium and 10% platelet-rich plasma-thrombin as the control groups, and 0.

Snakebite Treatment in Tanzania: Identifying Gaps in Community Practices and Hospital Resources.

Snakebite envenoming causes more than 140,000 deaths annually and at least triple this number of disabilities. The World Health Organization classified snakebite as a Neglected Tropical Disease in 2017 and developed a strategy to halve death and disability from snakebite by 2030. To achieve this goal, snakebite victims need to receive safe and effective treatment.

Changes in Migratory Speed Rate of Human Dental Pulp Stromal Cells Cultured in Advanced Platelet-Rich Fibrin.

Objective:  Migratory speed rate evaluation of human dental pulp stromal cells (hDP-SCs) is one of the important steps in dental pulp regeneration. Therefore, the aim of the study is to analyze various concentrations of advanced platelet-rich fibrin (A-PRF) culture media toward hDP-SCs' migratory speed rate evaluations.

Materials And Methods:  The hDP-SCs were divided into four groups: control: hDP-SCs in Dulbecco's modified Eagle medium + 10% fetal bovine serum group; hDP-SCs in 1% A-PRF group; hDP-SCs in 5% A-PRF group; and hDP-SCs in 10% A-PRF group, which were planted in 24-well (5 × 10 cell/well).

The Effect of Calcium Gluconate on Platelet Rich Plasma Activation for VEGF-A Expression of Human Dental Pulp Stem Cells.

Objective:  Platelet-rich plasma (PRP) activation is an important factor in triggering the initial release of blood-derived growth factors from platelets. Vascular endothelial growth factor-A (VEGF-A) can be expressed by human dental pulp stem cells (hDPSCs) and plays an important role in dental pulp angiogenesis. The aim of this study is to analyze the effects of calcium gluconate on PRP activation in hDPSC VEGF-A expression.

The Effect of Sr-CoFeO Nanoparticles with Different Particles Sized as Additives in CIP-Based Magnetorheological Fluid.

This study investigated the effect of adding strontium (Sr)-doped cobalt ferrite (CoFeO) nanoparticles in carbonyl iron particle (CIP)-based magnetorheological fluids (MRFs). Sr-CoFeO nanoparticles were fabricated at different particle sizes using co-precipitation at calcination temperatures of 300 and 400 °C. Field emission scanning electron microscopy (FESEM) was used to evaluate the morphology of the Sr-CoFeO nanoparticles, which were found to be spherical.

Health-promoting university: the implementation of an integrated guidance post for non-communicable diseases (Posbindu PTM) among university employees.

Non-communicable diseases (NCDs) remain a challenge globally and in Indonesia. Workplace environments may place employees at risk for NCD behavioral factors. This study aimed to develop an integrated guidance post for NCD (in Indonesian, 'pos pembinaan terpadu penyakit tidak menular' [Posbindu PTM] early detection among employees in one of the Indonesian universities.

Identification of active compounds in ethyl acetate, chloroform, and N-hexane extracts from peels of from Maribaya, West Java, Indonesia.

Lime peel contains essential oils are used as anti-oxidants or anti-cancer compounds. As a traditional medicine, lime has been widely used as a substitute for antibiotics. This study aimed to identify active compounds in peel extracts from that grows in Indonesia.

Extreme recurrent massive hemoptysis in a cystic fibrosis patient requiring 22 separate embolization procedures prior to lung transplantation: A case report.

While bronchial artery embolization is an established, safe, and effective treatment for massive hemoptysis from a variety of causes including cystic fibrosis, patients rarely require more than 2 angiography and embolization treatments during their lifetime. We present a rare case of massive, recurrent hemoptysis requiring a total of 22 angiography and embolization procedures over a period of 8 years, prior to the patient receiving a double lung transplant.

Inverse association of vitamin D levels with lung cancer mediated by genetic variation.

Vitamin D is an essential micronutrient required for normal physiological function and recognized for its role regulating calcium metabolism. Recent work is beginning to emerge demonstrating a role for vitamin D in chronic illnesses, such as cancer. Circulating serum levels of 25(OH)D were quantitatively measured using sensitive ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) in 406 lung cancer cases and 437 population controls, while 1,25(OH) D levels were measured in a subset of 90 cases and 104 controls using the same method, from the NCI-MD case-control cohort.

Efficacy according to blind independent central review: Post-hoc analyses from the phase III, randomized, multicenter, IPASS study of first-line gefitinib versus carboplatin/paclitaxel in Asian patients with EGFR mutation-positive advanced NSCLC.

Objective: The Phase III, randomized, open-label IPASS study (NCT00322452) of first-line epidermal growth factor receptor tyrosine kinase inhibitor (EGFR TKI) gefitinib versus carboplatin/paclitaxel for Asian patients with advanced non-small-cell lung cancer (NSCLC) showed that investigator-assessed progression-free survival (PFS) and objective response rate (ORR) were significantly prolonged in patients with EGFR mutation-positive NSCLC who received gefitinib versus patients with EGFR mutation-negative NSCLC. We report post-hoc analyses of IPASS data by blind independent central review (BICR), performed at the request of the US FDA, in a subset of patients with EGFR mutation-positive NSCLC.

Patients And Methods: Eligible patients (aged ≥18 years; histologically/cytologically confirmed Stage IIB/IV adenocarcinoma NSCLC; non- or former light-smokers; treatment-naïve) were randomly assigned 1:1 to gefitinib (250mg/day) or carboplatin (dose calculated to produce an area under the curve of 5 or 6 mg/mL/minute)/paclitaxel (200mg/m).

Urinary Metabolite Risk Biomarkers of Lung Cancer: A Prospective Cohort Study.

Background: Lung cancer is a major health burden causing 160,000 and 1.6 million deaths annually in the United States and worldwide, respectively.

Methods: While seeking to identify stable and reproducible biomarkers in noninvasively collected biofluids, we assessed whether previously identified metabolite urinary lung cancer biomarkers, creatine riboside (CR), N-acetylneuraminic acid (NANA), cortisol sulfate, and indeterminate metabolite 561+, were elevated in the urines of subjects prior to lung cancer diagnosis in a well-characterized prospective Southern Community Cohort Study (SCCS).

Can carbon emissions from tropical deforestation drop by 50% in 5 years?

Halving carbon emissions from tropical deforestation by 2020 could help bring the international community closer to the agreed goal of <2 degree increase in global average temperature change and is consistent with a target set last year by the governments, corporations, indigenous peoples' organizations and non-governmental organizations that signed the New York Declaration on Forests (NYDF). We assemble and refine a robust dataset to establish a 2001-2013 benchmark for average annual carbon emissions from gross tropical deforestation at 2.270 Gt CO2 yr(-1).

Global, regional, and national comparative risk assessment of 79 behavioural, environmental and occupational, and metabolic risks or clusters of risks in 188 countries, 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013.

Background: The Global Burden of Disease, Injuries, and Risk Factor study 2013 (GBD 2013) is the first of a series of annual updates of the GBD. Risk factor quantification, particularly of modifiable risk factors, can help to identify emerging threats to population health and opportunities for prevention. The GBD 2013 provides a timely opportunity to update the comparative risk assessment with new data for exposure, relative risks, and evidence on the appropriate counterfactual risk distribution.

Global, regional, and national disability-adjusted life years (DALYs) for 306 diseases and injuries and healthy life expectancy (HALE) for 188 countries, 1990-2013: quantifying the epidemiological transition.

Background: The Global Burden of Disease Study 2013 (GBD 2013) aims to bring together all available epidemiological data using a coherent measurement framework, standardised estimation methods, and transparent data sources to enable comparisons of health loss over time and across causes, age-sex groups, and countries. The GBD can be used to generate summary measures such as disability-adjusted life-years (DALYs) and healthy life expectancy (HALE) that make possible comparative assessments of broad epidemiological patterns across countries and time. These summary measures can also be used to quantify the component of variation in epidemiology that is related to sociodemographic development.

Detection and Dynamic Changes of EGFR Mutations from Circulating Tumor DNA as a Predictor of Survival Outcomes in NSCLC Patients Treated with First-line Intercalated Erlotinib and Chemotherapy.

Purpose: Blood-based circulating-free (cf) tumor DNA may be an alternative to tissue-based EGFR mutation testing in NSCLC. This exploratory analysis compares matched tumor and blood samples from the FASTACT-2 study.

Experimental Design: Patients were randomized to receive six cycles of gemcitabine/platinum plus sequential erlotinib or placebo.