Long-read sequencing of hundreds of diverse brains provides insight into the impact of structural variation on gene expression and DNA methylation.

Structural variants (SVs) drive gene expression in the human brain and are causative of many neurological conditions. However, most existing genetic studies have been based on short-read sequencing methods, which capture fewer than half of the SVs present in any one individual. Long-read sequencing (LRS) enhances our ability to detect disease-associated and functionally relevant structural variants (SVs); however, its application in large-scale genomic studies has been limited by challenges in sample preparation and high costs.

Pathogens spread by high-altitude windborne mosquitoes.

Recent studies have revealed that many mosquito species regularly engage in high-altitude windborne migration, but its epidemiological significance was debated. The hypothesis that high-altitude mosquitoes spread pathogens over large distances has not been directly tested. Here, we report for the first time that high-altitude windborne mosquitoes are commonly infected with arboviruses, protozoans, and helminths affecting vertebrates and humans, and provide the first description of this pathogen-vector aerial network.

Anthelmintic activity of crude and separated extract of (Xanthorrhoeaceae) against bovine adults parasites of and infected larvae of drug resistant strains of the free-living nematode .

Human onchocerciasis is caused by the filarial worm. is a parasite that forms nodules under the skin. The aim of this work was to assess the nematicidal activities of on and and to determine the phytochemical compounds.

Characterization of Lactic Acid Bacteria Isolated From Rotting Oranges and Use of Agropastoral Processing By-products as Carbon and Nitrogen Sources Alternative for Lactic Acid Production.

This study investigated the ability of lactic acid bacteria (LAB) isolated from oranges to use fish by-products (FB) and chicken by-products (CB) as nitrogen sources alternative to yeast extract for lactic acid (LA) production in a papaya by-product medium as a carbon source. Once the fermentation agents had been isolated, they were subjected to biochemical and molecular characterization. Inexpensive nitrogen sources, precisely CB and FB, were prepared, freeze-dried, and yield evaluated.

Diversity of African fungi, chemical constituents and biological activities.

Besides plants and animals, the fungal kingdom consists of several species characterized by various forms and applications. Fungi are amazing producers of bioactive natural products with applications in medicine and agriculture. Though this kingdom has been extensively investigated worldwide, it remains relatively underexplored in Africa.

2D:4D digit ratio as a potential marker for prostate cancer risk.

Background: The second-to-fourth digit ratio (2D:4D) is thought to reflect prenatal exposure to sex steroids. We investigated the relationship between 2D:4D and odds of prostate cancer.

Method: Data were collected in PROtEuS, a population-based case-control study conducted in Montréal, Canada (2005-2012), including 1931 incident prostate cancer cases aged < 76 years and 1994 population controls.

Identifying critical growth stage and resilient genotypes in cowpea under drought stress contributes to enhancing crop tolerance for improvement and adaptation in Cameroon.

Drought stress following climate change is likely a scenario that will have to face crop growers in tropical regions. In mitigating this constraint, the best option should be the selection and use of resilient varieties that can withstand drought threats. Therefore, a pot experiment was conducted under greenhouse conditions at the Research and Teaching Farm of the Faculty of Agronomy and Agricultural Sciences of the University of Dschang.

Experimental Investigation on the Effects of Tool Geometry and Cutting Conditions on Machining Behavior during Edge Finishing of Granite Using Concave and Chamfered Profiling Tools.

Granite edge finishing through grinding is a common process in the granite processing industry, crucial for achieving the final desired shape and edge quality of products. This study focuses on the granite industry, specifically delving into the significance of grinding and polishing for improving aesthetics and extending material longevity. The experimental design entails a comprehensive factorial experiment plan involving two workpiece materials (white and black granite samples) and two cutting tool edge shapes (chamfer and concave), each with two grit sizes: G150 and G600.

Determinants and constraints of carrot (Daucus carota L.) production and marketing in Cameroon.

The market gardening sector in Cameroonian agriculture is facing a challenge in producing vegetables to meet consumer needs. Among these vegetables, carrot is known to play an important role in the livelihood of millions of people around the world. It is therefore important to understand its technical itinerary in the different agricultural basins in order to provide further information on carrot production.

Estimation of diagnostic reference levels for pediatric head computed tomography in Yaoundé.

The diagnostic reference levels (DRLs) are determined from dosimetry studies to compare and manage patient doses in medical imaging procedures. The aim of this work was to establish the first DRLs for pediatric patients during the most common computed tomography (CT) procedures in Yaoundé, Cameroon during routine head CT examinations to know how to improve our clinical practice tomorrow. The sample was classified per age group: ≤ 1-, 1-5-, 5-10- and 10-15-y-old.

The African mosquito-borne diseasosome: geographical patterns, range expansion and future disease emergence.

Mosquito-borne diseases (MBDs) threaten public health and food security globally. We provide the first biogeographic description of the African mosquito fauna (677 species) and the 151 mosquito-borne pathogens (MBPs) they transmit. While mosquito species richness agrees with expectations based on Africa's land surface, African arboviruses and mammalian plasmodia are more speciose than expected.

The genus Canthium: A comprehensive summary on its traditional use, phytochemistry, and pharmacological activities.

Canthium Lam. is a genus of flowering plants of the Rubiaceae family with about 80-102 species mainly distributed in Asia, tropical and subtropical Africa. The genus is closely related to Keetia E.

Secondary metabolites of , isolated from the Cameroonian medicinal plant .

From a fresh root of (Ulmaceae), endophytic fungi were isolated, among which a taxon belonging to the new species . This strain was fermented in shake flask batch cultures and the broth was extracted with ethyl acetate. From the crude extract, a hemiketal polyketide , and an acetylated alternariol were isolated, along with fifteen known secondary metabolites.

Management of Atypical Teratoid/Rhabdoid Tumors in the Pediatric Population: A Systematic Review and Meta-Analysis.

Background: Atypical teratoid/rhabdoid tumors are rare and aggressive tumors that mainly affect children <3 years of age. Despite aggressive treatment, the overall survival rate for pediatric atypical teratoid/rhabdoid tumors remains poor. Due to their rarity, little is known regarding prognostic factors, and there is no official standard of treatment.

Polyoxygenated Stigmastane-Type Steroids from Sch. Bip. ex Walp. and Their Chemophenetic Significance.

Four polyoxygenated stigmastanes (-) alongside known analogues (-) and flavonoids (-) were isolated from a dichloromethane/methanol (1:1, /) extract of the whole plant of Sch. Bip. ex Walp.

Novel Approach to Percutaneous Lumbar Surgeries via Kambin's Triangle-Radiographic and Surgical Planning Analysis with Nerve Segmentation Technology.

Objective: While Kambin's Triangle has become an ever more important anatomic window given its proximity to the exiting nerve root, there have been limited studies examining the effect of disease on the corridor. Our goal was to better understand how pathology can affect Kambin's Triangle, thereby altering the laterality of approach for percutaneous lumbar interbody fusion (percLIF).

Methods: The authors performed a single-center retrospective review of patients evaluated for percLIF.

UPLC-QToF-ESI-MS identification and anthelmintic activity of (Willd.) Kuntze (Rubiaceae).

Medicinal plants attract the attention of many researchers to find natural and safe remedies for various resistant diseases. Leaves of are widely used in traditional veterinary medicine for the treatment of gastrointestinal strongyles of small ruminants. The aim of the current study is to estimate the antioxidant, anthelmintic and the larval toxicity of the aqueous and hydroethanolic extracts of this plant in addition to the hexane, dichloromethane and ethanol fractions of the hydroethanolic extract.

Comparative assessment of hepatoprotective properties of Artesunate and flavonoids from on acetaminophen and carbon tetrachloride-induced cytotoxicity in primary mice hepatocytes.

Background: Artesunate (ART) is a semi-synthetized molecule from Artemisinin, an active compound isolated from the medicinal plant , widely used for the treatment of malaria. Previous studies reported that ART may exert a dual effect on the liver. Accordingly, this study investigated the potential protective action of ART against Acetaminophen (APAP) and Carbon tetrachloride (CCl)-induced hepatotoxicity in primary mice hepatocytes, in comparison to that of flavonoid extracted from (FAA).

Potentiation effect of mallotojaponin B on chloramphenicol and mode of action of combinations against Methicillin-resistant Staphylococcus aureus.

Staphylococcus aureus, the causative agent of many infectious diseases has developed resistance to many antibiotics, even chloramphenicol which was the essential antibiotic recommended for the treatment of bacterial infection. Thus, other alternatives to fight against S. aureus infections are necessary; and combinatory therapy of antibiotics with natural compounds is one of the approaches.