Preparation of mixed keratinocyte and melanocyte cultures from biopsy specimens of pigmented skin sites of patients with vitiligo.

A method for preparation and expansion of mixed keratinocyte and melanocyte culture from human skin biopsy specimens was developed. The culture contains two melanocyte types: derivatives of hair follicle stem cells and epidermal basal layer stem cells. Fibroblasts were completely eliminated after culturing in selective medium.

[Immobilization of Saccharomyces yeast cells on electret polyethylene films].

The method of electret-thermal analysis developed in dielectric physics has been applied to monitor bioelectric phenomena that accompany the immobilization of microorganisms on electret substrates. A spectrum of thermo stimulated currents for biofilms consisting of immobilized Saccharomyces cells has been obtained. The spectrum represents a halo in the region of 30-90 degrees C.

[Principles of treatment and military medical examination of the patients with indolent Non-Hodgkin's lymphomas].

Non-Hodgkin's lymphomas (NHL) are the heterogeneous group of lymphoproliferative malignancies with distinct morphological, immuno-histochemical and genetic criteria of diagnosis, different character of clinical course, sensitivity to different treatment methods and disease prognosis. The WHO classification (1997) encompasses all the lymphoproliferative neoplasms. The NHL can be divided into 2 prognostic groups: the indolent lymphomas and the aggressive non-Hodgkin's lymphomas.

[The prevention and treatment of suppurative-septic complications in patients with acute appendicitis].

In the experiment on mongrel dogs, the absorption of indigo carmine dye after its retroperitoneal administration with 10% dimexide solution into the lymphatic and venous systems was studied. More rapid delivery of a dye into the portal system, including the cases with portal hypertension, and into the lymphatic system was established. A method for retroperitoneal administration of antibiotics with 10% dimexide solution for prevention and treatment of purulent-septic complications in patients with acute appendicitis has been developed.

[Treatment of different variants of the mixed form of portal hypertension].

Among 425 patients who were under observation for portal hypertension 72 (16.9%) had its mixed form. Thrombosis of the portal vein in liver cirrhosis occurred in 6 patients.

[Surgical treatment of pancreatic cancer and major papilla of the duodenum].

Based on their experience with treatment of 70 patients with carcinoma of the pancreas the authors substantiate expedience of using their original methods of pancreatodigestive and biliodigestive anastomoses during pancreatoduodenal resections. Indications to using the methods of surgical correction in different categories of patients are described.

[Use of Nichrome suture material].

The suture material and the method of its application play an important role in the prevention of wound complications. With the use of Nichrome wire thread as the suture material in surgical interventions, the number of postoperative complications significantly was decreased. The Nichrome thread is also used expediently in operative interventions on patients with purulent diseases of the soft tissues, including cases after primary surgical treatment of purulent foci by carbon dioxide laser.

[The therapeutic efficacy of tigazon in treating dermatoses].

Forty patients with various dermatoses (psoriasis, ichthyosis, Darier's disease, discoid lupus erythematosis) were followed-up over the course of treatment with tigason, an aromatic retinoid. The effect was satisfactory in the majority of patients. Side effects were recorded in 16 cases.

[First experience of using autotransfusions of UV-irradiated oxygenated blood in patients with various allergic dermatoses].

This treatment modality has been used in the management of 47 patients with toxicoderma and eczema. No side effects or complications have been observed. The eauthors analyze the literature data and their own findings concerning the mechanisms of action of such autotransfusions.

[Surgical tactics in the esophago-gastric hemorrhages of liver cirrhosis patients].

The character of the source and rate of bleeding were taken into consideration in determining the surgical tactics in 342 patients with cirrhosis of the liver complicated by gastro-esophageal bleedings and with high risk of their appearance. The operation of choice is thought to be arrest of the gastro-esophageal collateral blood flow. At high portal pressure or its considerable growing after the separating operation the latter should be associated with vascular anastomosis.