Publications by authors named "Kachanov OYu"
Biochemistry (Mosc)
April 1997
Article Synopsis
- The study used fluorescein isothiocyanate (FITC) to label coupled mitochondria and found that a significant portion of the dye is associated with mitochondrial proteins, particularly those within the inner membrane.
- FITC binding experiments suggest that mitochondrial proteins behave like polyelectrolytes, with a dissociation constant around 10(-7), and show competition between H+ and K+ ions for binding at neutral pH.
- The results also indicate that the deprotonation of matrix proteins occurs slowly and is linked to the equilibrium between matrix and medium protons, potentially affecting mitochondrial respiration rates under specific conditions.
