Simultaneous determination and pharmacokinetic study of giraldoid a, giraldoid B in rat plasma after oral administration of Daphne giraldii Nitsche extracts by LC-MS/MS.

A simple sensitive LC-MS/MS method has been developed for the simultaneous determination of giraldoid A and giraldoid B in rat plasma. The method was applied to pharmacokinetics studies of the two compounds from Daphne giraldii Nitsche. Chromatographic separation was accomplished on an Acquity UPLC™ BEH C column (100 × 2.

Chemical Constituents from Daphne giraldii Nitsche and Their Contents Simultaneous Determination by HPLC.

Daphne giraldii Nitsche (Thymelaeaceae) is widely distributed in the Chinese provinces of Shaanxi, Gansu, and Qinghai, which has been used in Chinese folk medicine to treat ache and rheumatism. Pharmacologic tests have revealed that the plant has anti-inflammatory, analgesic, and anticancer activities. However, there is still not enough systemic investigation on the chemical constituents and the method for the contents simultaneous determination in D.