Production of Esters in Using Citrate Synthase Variants.

Acetate esters comprise a wide range of products including fragrances and industrial solvents. Biosynthesis of esters offers a promising alternative to chemical synthesis because such routes use renewable carbohydrate resources and minimize the generation of waste. One biochemical method for ester formation relies on the gene from , which encodes alcohol-O-acyltransferase (AAT) which converts acetyl-CoA and an exogenously supplied alcohol into the ester.

Enhanced cellulosic ethanol production via fed-batch simultaneous saccharification and fermentation of sequential dilute acid-alkali pretreated sugarcane bagasse.

This study reports high gravity fed-batch simultaneous saccharification and fermentation (FB-SSF) of sequentially pretreated sugarcane bagasse (SCB) for enhanced bioethanol by employing multiple inhibitor tolerant Kluyveromyces marxianusJKH5 C60. FB-SSF with intermittent feeding of SCB (total 20 % solid loading) and enzyme (total dose of 20 FPU/g) at 6 and 12 h resulted in superior bioethanol production at42 °C. Under optimizedlab-scaleFB-SSF, the maximum ethanoltiter, efficiency and productivities were73.

Strategies to enhance enzymatic hydrolysis of lignocellulosic biomass for biorefinery applications: A review.

Global interest in lignocellulosic biorefineries has increased in the recent past due to technological advancements in sustainable and cost-effective production of numerous commodity and speciality chemicals and fuels from renewable lignocellulosic biomass (LCB). As a result, the market value of biorefinery products has also increased over the time, with an estimated worth of USD 867.7 billion by 2025.

Development of multiple inhibitor tolerant yeast via adaptive laboratory evolution for sustainable bioethanol production.

The present research work aimed at developing robust yeast cell factory via adaptive laboratory evolution (ALE) for improved cellulosic bioethanol production. Kluyveromyces marxianus JKH5, a newly isolated thermotolerant ethanologenic yeast, was engineered by serial passaging for 60 generations in medium supplemented with gradually higher concentration of inhibitors (acetic acid, furfural, and vanillin) that are generated during dilute acid pretreatment. The improved strain K.

Magnetically recyclable catalytic nanoparticles grafted with Bacillus subtilis β-glucosidase for efficient cellobiose hydrolysis.

This study reports covalent immobilization of β-glucosidase (BGL) from Bacillus subtilis PS on magnetically recyclable iron nanoparticles for enhancing robustness, facile recovery and reuse of enzyme. Immobilized BGL iron nanoparticles (BGL-INPs) were characterized by various biophysical techniques viz. TEM, DLS, FTIR and CD spectroscopy.

G Quadruplex in Plants: A Ubiquitous Regulatory Element and Its Biological Relevance.

G quadruplexes (G4) are higher-order DNA and RNA secondary structures formed by G-rich sequences that are built around tetrads of hydrogen-bonded guanine bases. Potential G4 quadruplex sequences have been identified in G-rich eukaryotic non-telomeric and telomeric genomic regions. Upon function, G4 formation is known to involve in chromatin remodeling, gene regulation and has been associated with genomic instability, genetic diseases and cancer progression.