Antigenic analysis of Cysticercus cellulosae by crossed immunoelectrophoresis & its role in immune diagnosis of neurocysticercosis.

The antigenic composition of Cysticercus cellulosae cysts excised from infected pig and autopsied human brain was analysed by crossed immunoelectrophoresis with an intermediate gel technique using rabbit hyperimmune serum. Normal pork muscle and human brain antigen were used to differentiate parasite derived components from that of host. Attempts were made to look for the rich source of parasitic immunodominant antigens by analysing preparations of different parts of cyst namely scolex and fluid using rabbit hyperimmune serum.

The far cry of a TB brain. Report of a case of tuberculous meningitis, multiple tuberculomas and tuberculous abscess.

An unusual case of neurotuberculosis presenting with tuberculous meningitis, tuberculous abscess and multiple tuberculomas is reported. The patient showed a dramatic response and resolution of tuberculomas with antituberculous therapy only after excision of the abscess.

Respiratory-competent conditional developmental mutant of Mucor racemosus.

A conditional developmental mutant of Mucor racemosus which is capable of oxidative energy metabolism is described. Unlike the wild-type strain the mutant was highly fermentative and exhibited the yeast morphology when grown aerobically in glucose-containing media. The high fermentative activity and yeast morphology under these conditions correlated well with maximal expression of glycolytic enzymes and with expression of some polypeptides characteristic of anaerobic growth.

Organization of the colicin Ib gene. Promoter structure and immunity domain.

The colicin Ib gene of the large low-copy-number plasmid Col Ib-P9 has been cloned into the plasmid vector pBR322. Observations of growth characteristics of strains carrying the cloned gene and analysis of insertion and deletion derivatives of the recombinant plasmid have led to the conclusion that colicin Ib production can occur without the colicin Ib immunity system. The direction of transcription for the colicin Ib gene was determined by use of an expression vector and subsequently confirmed by DNA-sequence analysis of the colicin Ib promoter.