[A comparative study of the synthesis of membrane hetero-organic antigens of kidney origin on tissue slices and isolated cells after a single carcinogenic exposure in rats].

A comparative study was carried out of a single carcinogenic injection of rats with a "weak" carcinogen, 4-dimethylaminoazobenzene (DAB), and a "strong" carcinogen, N-diethylnitrozamine (DENA). Both carcinogenic agents caused similar antigenic rearrangements involving the appearance of membrane hetero-organic antigens of kidney origin associated with the Zajdela hepatoma on the hepatocyte membrane. The expression of these antigens is longer in DENA carcinogenesis.

[The expression of hetero-organic antigens of kidney origin on the surfaces of cultured rat hepatocytes under the influence of the narrow-band fractions of non-histone chromosomal proteins].

Narrow fractions of nonhistone chromosomal proteins (NHCP) eluted with 0.4-0.5 M NaCl from the phosphocellulose column stimulate expression of hetero-organic antigens of kidney origin on the membrane of intact hepatocytes cultured in suspension.

[The effect of antithymocyte serum on the natural cytotoxicity of C3HA mouse splenocytes in the early periods after 20-methylcholanthrene administration].

The role of T-lymphocytes in natural cytotoxicity of splenocytes of C3HA mice after a single injection of 20-methylcholanthrene (20-MC) was investigated. A splenocyte suspension was treated with anti-T-cell serum and complement. This treatment was not shown to exert influence on the natural cytotoxicity of splenocytes within 1-13 days after injecting 20-MC.

[Activity of natural killer cells in various forms of abortion].

The activity of natural killer (NK) cells was found to be significantly higher in females with various miscarriages than that in those with normal pregnancy. The highest NK cell activity was observed in females with interrupted pregnancy.

[The isolation and characteristics of monoclonal antibodies to the HNK-1 antigen of natural killers].

Monoclonal antibodies to the HNK-1 differentiated antigen of natural killer cells have been obtained. A glycoprotein of the white human brain connected with myelin was used as an antigen for immunization of mice. The monoclonal antibodies obtained are shown to reduce the cytotoxic activity of a human blood mononuclear fraction as much as by 65% in relation to the human lymphoblastoma cell culture K-562.

[The stimulation of DNA synthesis in resting Swiss 3T3 cells by non-histone chromosomal proteins].

Nonhistone chromosomal proteins (NHC), isolated from the kidney and liver of intact rats, the liver of rats treated with hepatocarcinogen DEN and the rat hepatoma, stimulate DNA synthesis in Swiss 3T3 cells in resting culture. The maximum stimulating effect was obtained in the presence of narrow NHC fractions eluted with 0.4-0.

[The antigenic divergence of tumor cells due to the expression of hetero-organic antigens as a manifestation of disordered differentiation in carcinogenesis].

The analysis has been made of the literary and the author's own experimental evidences on the antigenic diversion of tumor cells resulting from the expression of hetero-organic antigens, which is one of the most characteristic manifestations of disturbance of cytodifferentiation in carcinogenesis. Studies of hepatocellular tumors and liver of rats subject to single hepatocarcinogen injections being exemplified, the author considers some cases of detection of hetero-organic antigens of kidney origin in the cytosol, on the outer cell membranes and within the chromosomal non-histone proteins (NHP). Under discussion is the interrelation between the expression of hetero-organic antigens, attributed to the same tissue type in different cell structures, and a possible role of NHP as factors directing the disdifferentiation of neoplastically transformed cells.

[The immunomodulation of the natural killer activity of the splenocytes in C3HA mice during hepatoma 22a growth].

A single injection of C3HA mice with various immunomodulators-ds-RNA, thymogene (TM) and cyclophosphamide (CY)--performed one day before transplantation of syngeneic hepatoma 22a cells led to a decrease in the tumor growth rate. The most prominent effect was found following the CY treatment. The NK cell activity estimated per spleen of mice treated with ds-RNA and TM was seen increased in comparison with the control mice not given the modulators.

[The effect of preparations of non-histone chromosomal proteins on the expression of hetero-organic membrane antigens of kidney origin in a primary rat hepatocyte culture].

The expression of membrane hetero-organic antigens of kidney origin, associated with the rat hepatomas in primary culture of intact adult rat hepatocytes, was investigated by means of the indirect immunofluorescence method using a specific immune serum. These antigens were observed on the membrane of some hepatocytes after their contact with nonhistone chromosomal proteins (NHCP), which were obtained from the kidney of intact rats from cells of hepatoma 27 and Zajdela hepatoma, or from the carcinogenic liver after a single diethylnitrosamine injection. Negative results were obtained after the incubation of hepatocytes in the medium lacking some of NHCP, or in that with NHCP obtained from the liver of intact rats.

[The effect of anti-T sera on the natural killer activity of the splenocytes in C3HA mice at different times after the transplantation of hepatoma 22a cells].

Suspension of splenocytes of control C3HA mice and mice examined early after transplantation of mouse hepatoma 22a cells were fractionated by treatment with anti-T-sera (anti-thymocytes, antibrain and anti-suppressor-T-cells). This treatment leads to various changes in NK-activity due to elimination of different subpopulations of T-lymphocytes. This variability may be associated with the presence of two or more types of suppressor cells able to influence the NK-cells directly or by means of other immunocompetent cells.

[Antigenic divergence in a primary rat hepatocyte culture after exposure to the hepatic carcinogen N-diethylnitrosamine].

Using the indirect immunofluorescence method, the appearance of membrane hetero-organic antigens of kidney origin associated with the Zajdela hepatoma cells was observed on the surface of cells of the rat liver primary culture following a single hepatocarcinogen N-diethylnitrosamine (DENA) treatment before and after explantation. A correlation of antigenic alterations after a single DENA treatment in vivo and in vitro was discovered. No antigens under investigation were discovered in cultured hepatocytes of intact adult rats.

[Natural killer activity of the splenocytes in C3HA strain mice during 20-methylcholanthrene-induced carcinogenesis].

The induction of tumor in C3HA mice after intramuscular injection with 20-methylcholanthrene is accompanied by a decrease in natural antitumor resistance. This conclusion is based on the observation of the decreased natural killer activity per total number of splenocytes, from the time of carcinogen application till the appearance of tumor nodes.

[The regulation of splenocyte natural killer activity in C3HA mice in the early period after hepatoma 22a transplantation].

A decrease of the natural killer activity (NKA) of C3HA splenocytes after transplantation of hepatoma 22a cells was connected to some extent with the suppressor action of macrophages and T-lymphocytes. Elimination of the macrophages from the splenocyte suspension led to the increase of NKA. Addition of the macrophages to the nonadherent cells resulted in the NKA inhibition to the level of nonfractionated splenocytes.

[Radiometric detection of nonvisualizable immunoprecipitates formed by hepatoma-associated hetero-organic renal antigens of 33P-phosphorylated fractions of non-histone chromatin proteins].

A study was made of the antigenic properties of nuclear nonhistone proteins (NHP) from rat tissue eluted with 0.4-0.5 M NaCl gradient on phosphocellulose at very low concentrations.

[Natural killers in pregnancy: the problem of heterogeneity and the regulation of activity].

Natural killer activity of pregnant women's peripheral blood lymphocytes and of cord blood was investigated. 3H-uridine labeled K-562 and human embryo fibroblasts (HEF) were used as target cells in cytotoxic test. The results of competitive inhibition test led us to a conclusion about the presence of some common K-562 and HEF surface structures recognized by NK cells.

[Characteristics of the changes in the natural killer activity of peripheral blood lymphocytes during pregnancy].

The influence of blood plasma of women of the 1st and 3rd pregnancy trimester and of cord blood on natural killer (NK) activity of peripheral blood lymphocytes of non-pregnant women was investigated. The maximum suppressive activity on NK was observed with plasma of pregnant women of the 3rd trimester with low NK-activity. Data suggesting the involvement of estradiol in this suppressive activity was obtained.

[Characteristics of the morphofunctional changes in the liver of C3HA mice due to immunization with a homogenate of normal syngeneic liver].

Immunization of C3HA mice with homogenate of normal syngeneic liver at a dosage causing elevation of antitumor resistance damages the liver of recipients. The damage involves the formation of foci of necrosis and the alteration in activities of some tissue specific and embryonal enzymes. The damage is reversible; its degree and the rate of reverse depends on a dosage of homogenate injected.

[Natural killer activity of splenocytes of C3HA mice in the early period after transplantation of hepatoma 22a cells].

It is shown that during 10 days after transplantation of syngenic 22a hepatoma cells to C3HA mice, wave-like fluctuations of the natural killer cell (NK) activity of splenocytes take place. Xenogenic (K-562) and allogenic (AC-1) cells are used as target cells in the 18th hour 3H-uridine cytotoxic test. A significant increase in the NK-activity on the 2nd-3rd and 7-9th days after tumour cells transplantation is observed.

[Heterogeneity of the effectors of natural killer activity in CBA mice].

The natural cytotoxicity of Percoll fractions of CBA mice splenocytes was tested against cells of human erythroblastosis suspension culture K-562 and murine C3HA hepatoma solid culture MGXXIIa. The 1.076 g/ml dense fraction was shown to have the highest activity in 3H-uridine cytotoxic test against the cell targets.

[Detection of cells resistant to the cytotoxic action of CCl4 in rat hepatocyte populations following a single exposure to diethylnitrosamine and subsequent injections of phenobarbital].

A single intraperitoneal injection of hepatocarcinogen diethylnitrosamine induced emergence of a new subpopulation of "small" hepatocytes (64-128 mkm2), disappearing by the 30th day after carcinogen injection. But 5 injections of the tumor promotor phenobarbital 7 days after carcinogen treatment prolonged the existence of such "small" hepatocytes up to 3 months. The quantitative cytochemical measurement of succinic dehydrogenase activity (respiratory enzyme of mitochondria) showed these cells to be resistant to cytotoxic action of CCl4.

[Mouse splenocyte sensitization to normal tissue antigens and natural killer activity. III. The effect of partial hepatectomy].

Partial hepatectomy leads to both increasing of natural cell-mediated activity and sensibilization level (SL) of splenocytes of hepatectomized mice towards antigens of the syngeneic liver. The wave-like variability of SL was shown with sharp increase at 3, 6 and 9 days after operation. Natural killer activity was elevated on the 2nd and 10th days with a significant decrease on the 3-4th days after operation.

[Hetero-organic antigens of renal nature in the composition of the nuclear nonhistone proteins in the regenerating rat liver].

In the nuclear non-histone proteins of the rat liver, on the 4th day after a partial hepatoectomy or hepatocarcinogen injection as well as in the hepatocellular tumour cells, some heteroorganic antigens of kidney nature (HAkid) are found and characterized immunochemically. These HAkid can be eluted at 0.4-0.