Development of two recombinase-aided amplification assays combined with lateral flow dipstick (RAA-LFD) and real-time fluorescence (RF-RAA) for the detection of Frog virus 3-like ranaviruses.

Frog virus 3-like ranaviruses (FV3-like viruses), particularly FV3 (Frog virus 3), represent typical species within the genus Ranavirus, primarily infecting amphibians and reptiles, thereby posing serious threats to aquaculture and biodiversity conservation. We designed a pair of universal primers and a probe targeting the conserved region of the major capsid protein (MCP) genes of FV3-like viruses. By integrating recombinase-aided amplification (RAA) with lateral flow dipstick (LFD) technology and real-time fluorescence (RF) modification, we established RAA-LFD and RF-RAA assays.

The availability of drugs for stable COPD treatment in China: a cross-sectional survey.

This survey aimed to investigate the availability of drugs for stable chronic obstructive pulmonary disease (COPD) treatment in Chinese hospitals and to determine whether drug availability significantly varied among hospitals with different characteristics. A well-constructed questionnaire was designed according to the Chinese Guidelines for the Diagnosis and Management of COPD (revised version 2021). Both inhaled drugs (monotherapy, double therapy and triple therapy) and oral drugs (expectorants, theophylline, antibiotics, and bacterial lysates) were included in this survey.

Subdiaphragmatic vagotomy reduces hypothalamic oxytocin expression and blood levels after oral MDMA administration in male rats.

3,4-Methylenedioxymethamphetamine (MDMA) is a widely recognized entactogen frequently used recreationally. It is known for its interaction with the serotonin and oxytocin systems, which underlie its entactogenic effects in humans. Recently, we demonstrated that the gut-brain axis, mediated by the subdiaphragmatic vagus nerve, contributes to MDMA-induced resilience enhancement in rodents.

Nanosheet-shaped WS/ICG nanocomposite for photodynamic/photothermal synergistic bacterial clearance and cutaneous regeneration on infectious wounds.

Bacterial infections present a significant threat to human health, a challenge that is intensified by the slow pace of novel antibiotic development and the swift emergence of bacterial resistance. The development of novel antibacterial agents is crucial. Indocyanine green (ICG), a widely used imaging dye, efficiently generates reactive oxygen species (ROS) and heat for treating bacterial infections but suffers from aggregation and instability, limiting its efficacy.

Effects of ratios of A- and B-type wheat starch on liangpi (a starch gel food) quality and its potential mechanism.

The fractionation and reconstitution method was employed to investigate the impact of different proportions of A-type (AS) and B-type (BS) wheat starch on the quality of liangpi and its potential mechanism, with traditional gluten-removed liangpi (RGL) as a control. The results demonstrated that a higher AS/BS ratio led to an increase in hardness, resilience, and chewiness. Moreover, the sensory scores for BS-dominated liangpi were significantly lower than those for AS-dominated liangpi, and liangpi with 60 % AS had the highest sensory scores.

Global comparison of the economic costs of coronary heart disease: a systematic review and meta-analysis.

Objectives: Coronary heart disease (CHD) is the leading cause of global morbidity and mortality, yet no comprehensive evaluation of its global economic costs exists. We conducted a systematic review with meta-analysis to examine the costs of CHD treatment by region and CHD subtypes, examine whether there are cost difference by sex, and examine costing methodologies.

Design: We conducted a systematic review and meta-analysis of non-randomised studies.

BTSegDiff: Brain tumor segmentation based on multimodal MRI Dynamically guided diffusion probability model.

In the treatment of brain tumors, accurate diagnosis and treatment heavily rely on reliable brain tumor segmentation, where multimodal Magnetic Resonance Imaging (MRI) plays a pivotal role by providing valuable complementary information. This integration significantly enhances the performance of brain tumor segmentation. However, due to the uneven grayscale distribution, irregular shapes, and significant size variations in brain tumor images, this task remains highly challenging.

Single-cell RNA-sequencing and spatial transcriptomic analysis reveal a distinct population of APOE cells yielding pathological lymph node metastasis in papillary thyroid cancer.

Background: Thyroid cancer is one of the most common endocrine tumors worldwide, especially among women and the metastatic mechanism of papillary thyroid carcinoma remains poorly understood.

Methods: Thyroid cancer tissue samples were obtained for single-cell RNA-sequencing and spatial transcriptomics, aiming to intratumoral and antimetastatic heterogeneity of advanced PTC. The functions of APOE in PTC cell proliferation and invasion were confirmed through in vivo and in vitro assays.

Regulation of Histone Acetylation Modification on Biosynthesis of Secondary Metabolites in Fungi.

The histone acetylation modification is a conservative post-translational epigenetic regulation in fungi. It includes acetylation and deacetylation at the lysine residues of histone, which are catalyzed by histone acetyltransferase (HAT) and deacetylase (HDAC), respectively. The histone acetylation modification plays crucial roles in fungal growth and development, environmental stress response, secondary metabolite (SM) biosynthesis, and pathogenicity.

Effects of pristine and photoaged tire wear particles and their leachable additives on key nitrogen removal processes and nitrous oxide accumulation in estuarine sediments.

Despite growing attention to the environmental pollution caused by tire wear particles (TWPs), the effects of pristine and photoaged TWPs (P-TWPs and A-TWPs) and their TWP leachates (TWPLs; P-TWPL and A-TWPL) on key nitrogen removal processes in estuarine sediments remain unclear. This study explores the responses of the denitrification rate, anammox rate, and nitrous oxide (NO) accumulation to P-TWP, A-TWP, P-TWPL, and A-TWPL exposure in estuarine sediments, and assesses the potential biotoxic substances present in TWPLs. P-TWPs reduced the denitrification rate by 17.

Effects of White Noise Intervention on Sleep Quality and Immunological Indicators of Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy.

Objective: Evaluate the effect of white noise intervention on sleep quality and immunological indicators of patients with breast cancer undergoing neoadjuvant chemotherapy (NAC).

Methods: From January 2020 to December 2022, 104 newly diagnosed female patients (the number of people who met the inclusion criteria) with breast cancer who were confirmed to be preoperative NAC by puncture pathology were selected for a randomised single-blind trial. The patients were randomly divided into an observation group and a control group, with 52 cases in each group.

A Preclinical Immunogenicity Study of the Recombinant Human Papillomavirus Nine-Valent Virus-like Particle Vaccine.

Background: Cervical cancer is associated with persistent infection of high-risk human papillomaviruses (HPVs). Prophylactic HPV vaccines have been recommended and have significant efficacy in preventing cervical cancer. Multivalent HPV vaccines have a better preventative effect on HPV-related diseases.

Promoting Healthy Aging for Older People Living with Chronic Disease by Implementing Community Health Programs: A Randomized Controlled Feasibility Study.

The rising prevalence of chronic diseases could be mitigated by expanding community programs. This study aimed to evaluate the feasibility of delivering a community wellness program for older adults living with chronic disease. A two-group randomized controlled study, with blinded assessments, enrolling adults (≥50 years) with chronic disease, was conducted at a Western Australian community hub.

Analysis of risk factors for abnormal renal function in patients with gout in Southwest China: a cross-sectional study.

Objective: To analyse the associations between renal function and clinical laboratory indicators and explore the renal function abnormality risk factors for gout patients in Southwest China.

Methods: Outpatient and hospitalized gout patients ( = 4384) at the First Affiliated Hospital of Chengdu Medical College between January 2017 and December 2020 were divided into normal ( = 2393) and abnormal ( = 1991) renal function groups according to their eGFR. The relationships between clinical laboratory indicators and the eGFR were analysed, and a logistic regression model was fit to identify significant risk factors.

The link between eye movements and cognitive function in mild to moderate Alzheimer's disease.

This study investigated the relationship between eye movement parameters and cognitive function in patients with mild to moderate Alzheimer's disease (AD). A total of 80 patients with AD (mild and moderate) and 34 normal controls (NC) participated. Neuropsychological assessments were conducted using the Mini-Mental State Examination (MMSE) and the Montreal Cognitive Assessment (MoCA), while eye movements were recorded using eye-tracking technology.

Lenalidomide regulates the CCL21/CCR7/ERK1/2 axis to inhibit migration and proliferation in diffuse large B-cell lymphoma.

Background: The prognostic significance of the chemokine receptor CCR7 in diffuse large B-cell lymphoma (DLBCL) has been reported previously. However, the detailed mechanisms of CCR7 in DLBCL, particularly regarding its interaction with lenalidomide treatment, are not fully understood.

Methods: Our study utilized bioinformatics approaches to identify hub genes in SU-DHL-2 cell lines treated with lenalidomide compared to control groups.

Design, Synthesis, and Fungicidal Activity of α-Methylene-γ-Butyrolactone Derivatives Bearing a Diphenyl Ether Moiety.

The γ-butyrolactone scaffold, commonly present in natural products and bioactive compounds, has played a crucial role in the development of novel pesticides. In this study, a series of α-methylene-γ-butyrolactone derivatives containing a diphenyl ether moiety were designed and synthesized using the scaffold splicing strategy. Bioassays revealed that several target compounds demonstrated potent fungicidal activities, particularly against and .

Arketamine alleviates cognitive impairments and demyelination in mice with postoperative cognitive dysfunction via TGF-β1 activation.

Postoperative cognitive dysfunction (POCD) is characterized by a decline in cognitive functions, including memory, attention, and executive abilities, following surgery, with no effective therapeutic drugs currently available. Arketamine, the (R)-enantiomer of ketamine, has shown promise in mitigating cognitive deficits in animal models. In this study, we investigated whether arketamine could ameliorate cognitive deficits in a mouse model of POCD, with a focus on the role of transforming growth factor (TGF)-β1 in its effects.

Chitosan hydrogel incorporated with bone marrow mesenchymal stem cell-derived exosomal TIMP2 to inhibit angiogenesis in cholangiocarcinoma.

Objective: Cholangiocarcinoma (CCA) presents a therapeutic challenge due to its aggressiveness and poor survival rates. This study introduces an approach using tissue inhibitor of metalloproteinase 2 (TIMP2)-enriched bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exo) encapsulated in chitosan hydrogels (CS), intending to provide novel insight into the CCA treatment.

Methods: BMSC-Exo was characterized by using TEM, nanoparticle tracking analysis, and western blotting.

Spatial heterogeneity determines the gastrointestinal microbiome signatures and ecological processes that govern bacterial community assembly in sheep.

Unlabelled: Sheep are one of the globally significant livestock, providing meat, dairy products, and wool for human life, playing an indispensable role in human civilization. Despite significant advancements in microbiome research in recent years, most studies have focused solely on the rumen, lacking a comprehensive study covering the microbiome of different gastrointestinal tract (GIT) regions in sheep. In this study, we collected 338 samples from 10 different regions of the sheep GIT and systematically investigated their microbiome signatures, including community structure, enterotypes, interactions among taxa, and microbial community assembly.

Two secretory T2 RNases from a fungal pathogen target distinct insect cell transmembrane proteins to cause cytotoxicity.

Fungal pathogens produce secretory ribonuclease (RNase) T2 proteins during infection, which contribute to fungal virulence via their enzyme functions in degradation of host cell RNA. However, the details of those proteins entering the host cells are unclear. Our previous study demonstrated that the two secretory RNase T2 members, BbRNT2 and BbTrv, produced by the insect fungal pathogen Beauveria bassiana, caused cytotoxic damage to insect cells and contributed to fungal virulence.

Effects of 3,4-methylenedioxymethamphetamine on the gut microbiota and metabolites in the small intestine, cecum, and colon of male rats.

3,4-Methylenedioxymethamphetamine (MDMA; Ecstasy) is a widely abused recreational drug that has also gained interest for potential clinical applications in mental health. With the growing recognition of gut microbiota's role in mental health, this study examined whether repeated oral MDMA administration could affect gut microbiota in the small intestine, cecum, and colon of male rats. Repeated oral MDMA administration (10 mg/kg/day for 14 days) caused significant changes in the gut microbiota across these regions, with distinct effects observed in each.

The Correlation Analysis Between m6A Methylation Modification and Ferroptosis Induced by Cigarette Smoke in Human Bronchial Epithelium.

Background: Chronic obstructive pulmonary disease (COPD), a prevalent respiratory condition, is characterized by long-term airway inflammation, which can lead to airway remodeling and persistent airflow restriction. Exposure to cigarette smoke is known as a major contributor to COPD development. Research has confirmed that ferroptosis and m6A modification are closely related to various inflammatory-related diseases.