Changes in the electrical properties of the skin outermost layer during pulse electrotreatment.

The kinetics of changes in the electric current I(t) passing through human skin samples of full thickness in vitro during rectangular voltage pulses (amplitude, 10-60 V; duration, 5-8 ms) was investigated. The function I(t) was shown to rapidly decrease, to pass through its minimum, and then to increase slowly. With the increase in voltage, the minimal current grew; the dropping branch became less pronounced (up to its complete disappearance at 40 V); and the position of the minimum shifted to short times.

A theoretical study of outermost skin layer electroporation.

Two models of electrohydration of stratum corneum (SC) have been developed. In the first model, the hydration of one interbilayer region is considered on the assumption that water molecules are adsorbed on the inhomogeneous surface of a bilayer and can interact, thereby lowering their energy on the surface. The dependence of the hydration degree on the voltage across the skin had been found.

Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores.

It has been shown recently that electrically induced DNA transfer into cells is a fast vectorial process with the same direction as DNA electrophoresis in an external electric field (Klenchin, V. A., S.

Electrically induced DNA uptake by cells is a fast process involving DNA electrophoresis.

Simian Cos-1 cells were transfected electrically with the plasmid pCH110 carrying the beta-galactosidase gene. The efficiency of transfection was determined by a transient expression of this gene. When the plasmid was introduced into a cell suspension 2 s after pulse application, the transfection efficiency was shown to be less than 1% as compared with a prepulse addition of DNA.

Electro-stimulated transformation of E. coli cells pretreated by EDTA solution.

The phenomenon of transformation of E. coli cells under electric treatment has been studied. The cells of strains MH 1, HB 101 and DH 1 after EDTA treatment in an isotonic medium were transformed with DNA pBR322 by applying a single exponential pulse (E = 10 kV/cm, T = 1.

Simulation of molecular dynamics of water movement in ion channels.

The motion of water molecules in a gramicidin-like channel was studied by the molecular dynamics method. Water molecules are presented in the ST2 model. The structure of the channel was presented in the form of channel's helix frame possessing mobile dipole groups.