Soil permeability shaping ARGs patterns by affecting soil available nutrients in paddy fields.

Though the evidence for soil property could influence the antibiotic resistance genes (ARGs) profiles is mounting, studies regarding the effect of soil permeability on soil ARGs patterns are still ignored. This study investigated the dynamic distribution of ARGs in paddy fields with different soil permeability over various rice growing stages, as well as revealed the abiotic and biotic factors that shaping ARGs profiles. Results indicate that soil with high permeability improved the ARGs abundance through elevating the available nutrients in the soil.

3D printed clear resin stir bar sorptive extraction coupled with high performance liquid chromatography for trace chlorophenols analysis in environmental water samples.

3D printing is an additive manufacturing technology based on digital model files. 3D printing has become a popular manufacturing tool in various fields. Stereolithography offers a series of advantages compared to its counterparts, such as smooth prints, appropriate resolution in all the axes, acceptable organic solvent compatibility and sufficient tightness to the flowing of solutions/solvents at moderate/high pressure.

Optimizing Shoulder Joint Positioning During Video-Assisted Thoracoscopic Surgery: A Prospective Study on Prevention of Postoperative Ipsilateral Shoulder Pain.

Background: Ipsilateral shoulder pain (ISP) is a common complication following video-assisted thoracoscopic surgery (VATS), significantly affecting postoperative recovery and quality of life. This study aimed to evaluate the relationship between intraoperative upper limb positioning, and the occurrence of ISP, with the goal of optimizing positioning to reduce postoperative complications.

Methods: This prospective cohort study included 252 patients undergoing VATS for lung resection.

Adult Kerion Caused by Trichophyton tonsurans.

Adult tinea capitis, especially kerion, caused by Trichophyton tonsurans is relatively rare in China. Here, we report a case caused by the agent in an old woman with normal immune function. Fungal microscopic examination and culture were positive.

NAT10 promotes pyroptosis and pancreatic injury of severe acute pancreatitis through ac4C modification of NLRP3.

Severe acute pancreatitis (SAP) is a highly morbid acute digestive disorder linked to pyroptosis. N-acetyltransferase 10 (NAT10) facilitates the production of N4-acetylcytidine (ac4C) modifications in mRNA, thereby contributing to the progression of various diseases. However, the specific role of NAT10 in SAP remains to be elucidated.

Research on the impact of mutual elderly care on the physical and mental health of rural elderly in China.

To study the impact of mutual care on the physical and mental health of rural elderly, and provide countermeasures and suggestions for the long-term development of mutual care. Data chosen from the 2018 China health and retirement longitudinal study (CHARLS) were selected, with 8369 elderly people aged ≥ 60 years old, and propensity scores were matched using nearest-neighbor matching, kernel matching, and radius matching, and then heterogeneity was used to analyze the mean effect values among different populations. The results of propensity score matching PSM showed that participation in mutual care had a significant positive effect on the mental health of the rural elderly, with a net effect of 0.

Evaluating a clinically available artificial intelligence model for intracranial aneurysm detection: a multi-reader study and algorithmic audit.

Purpose: We aimed to validate a clinically available artificial intelligence (AI) model to assist general radiologists in the detection of intracranial aneurysm (IA) in a multi-reader multi-case (MRMC) study, and to explore its performance in routine clinical settings.

Methods: Two distinct cohorts of head CT angiography (CTA) data were assembled to validate an AI model. Cohort 1, comprising gold-standard consecutive CTA cases, was used in an MRMC study involving six board-certified general radiologists.

An Outbreak of Foodborne Botulism Caused by BoNT/A3 in Pickled Eggs - Weihai City, Shandong Province, China, July 2024.

What Is Already Known About This Topic?: Foodborne botulism is caused by botulinum neurotoxin (BoNT). () is a strictly anaerobic, Gram-positive bacterium, which is a key pathogen capable of producing BoNT. BoNTs can be classified into seven serotypes (A to G) based on their antigenic properties.

Radiative Warming Glass for High-Latitude Cold Regions.

Traditional window glazing, with inherently adverse energy-efficient optical properties, leads to colossal energy losses. Energy-saving glass requires a customized optical design for different climate zones. Compared with the widely researched radiative cooling technology which is preferable to be used in low-altitude hot regions; conversely in high-latitude cold regions, high solar transmittance (T) and low mid-infrared thermal emissivity (ε) are the key characteristics of high-performance radiative warming window glass, while the current low-emissivity (low-e) glass is far from ideal.

Impurity detection of premium green tea based on improved lightweight deep learning model.

Tea may be mixed with impurities during picking and processing, which can lower their quality. At present, the sorting of impurities in premium green tea mainly relies on manual labor, which is inefficient. In response to the technical challenges in this industry, this article uses deep learning technology to detect impurities in premium green tea.

The swelling mechanism of ethylene-vinyl acetate polymer in different solvents molecular dynamics and experimental studies.

Ethylene-vinyl acetate (EVA) film is the predominant encapsulation material in crystalline silicon photovoltaic modules, the efficient and eco-friendly processing of which is essential for the recycling of the modules. Among the various existing techniques, the chemical approach uses solvents to induce swelling and dissolution on the EVA film to facilitate the separation of distinct layers. This method demonstrates the potential for achieving low-energy consumption and minimal-damage retrieval of the diverse materials within the components.

Effects of personalized 3D-printed blocks in total knee arthroplasty and revision surgery for massive bone defects: a single-center retrospective study.

Objective: To analyze the early- to mid-term clinical efficacy of personalized 3D-printed structural metal spacer technology in reconstructing massive bone defects during complex total knee arthroplasty (TKA) and revision surgery.

Methods: A single-center retrospective study was conducted on nine patients with severe bone defects who underwent TKA between 2018 and 2024. The general condition, surgical details, and clinical improvement of these patients were recorded and analyzed by clinical doctors.

Discovery of INCB159020, an Orally Bioavailable KRAS G12D Inhibitor.

The inhibition of mutant KRAS proteins has emerged as a promising approach for treating KRAS-driven cancers, as evidenced by the clinical success of KRAS G12C inhibitors. KRAS G12D, the most common mutant, promises significant expansion of the addressable patient population; however, the reduced nucleophilicity of aspartate compared to cysteine poses significant challenges in balancing sufficient potency with ADME properties to support oral exposure. Herein, we describe the discovery of KRAS G12D inhibitor (), which achieves oral exposure in nonhuman primate (NHP).

Effects of Phenoxazine Chromophore on Optical, Electrochemical and Electrochromic Behaviors of Carbazole-Thiophene Derivatives.

Phenoxazine, as an organic-small-molecule chromophore, has attracted much attention for its potential electrochromic applications recently. To develop appealing materials, phenoxazine chromophores were introduced at the N-position of carbazole-thiophene pigment, yielding two novel monomers (DTCP and DDCP), whose chemical structures were characterized by NMR, HRMS and FTIR. The results of the optical property study indicate that little influence could be observed in the presence of the phenoxazine chromophore.

Design and Experiment of a PLC-Based Intelligent Thermal Insulation Box for Nursing Piglets.

Local heating of the activity area for nursing piglets is crucial for piglet health and the energy efficiency of barn climate control. Traditional heating methods using lamps or covers lack precise control, result in significant energy waste, and cannot be dynamically adjusted according to piglet age or changing environmental temperatures. To address these issues, this study designed a Programmable Logic Controller (PLC)-based thermal insulation box for nursing piglets, utilizing a strip heater instead of the conventional round heating lamp.

Conference 2024: Building an innovative scientific research ecosystem for microbiome and One Health.

The Conference 2024 provides a platform to promote the development of an innovative scientific research ecosystem for microbiome and One Health. The four key components - Technology, Research (Biology), Academic journals, and Social media - form a synergistic ecosystem. Advanced technologies drive biological research, which generates novel insights that are disseminated through academic journals.

Development and validation of a nomogram for predicting the risk of obstructive coronary artery disease in rheumatoid arthritis patients based on LDL-C, Th17 cells, and IL-17.

Objective: This study aims to develop and validate a nomogram model for predicting the risk of obstructive coronary artery disease (CAD) in patients with rheumatoid arthritis (RA), incorporating low-density lipoprotein cholesterol (LDL-C), Th17 cells, and interleukin (IL)-17 levels. The proposed model seeks to enable personalized cardiovascular risk assessment for RA patients, thereby optimizing clinical management strategies.

Methods: A total of 120 patients with rheumatoid arthritis (RA) who were treated at the Second Hospital of Shanxi Medical University between January 2019 and September 2023 were enrolled in this study.

The miR-23a/27a/24 - 2 cluster drives immune evasion and resistance to PD-1/PD-L1 blockade in non-small cell lung cancer.

Programmed cell death protein ligand-1 (PD-L1) and major histocompatibility complex I (MHC-I) are key molecules related to tumor immune evasion and resistance to programmed cell death protein 1 (PD-1)/PD-L1 blockade. Here, we demonstrated that the upregulation of all miRNAs in the miR-23a/27a/24 - 2 cluster was correlated with poor survival, immune evasion and PD-1/PD-L1 blockade resistance in patients with non-small cell lung cancer (NSCLC). The overexpression of all miRNAs in the miR-23a/27a/24 - 2 cluster upregulated PD-L1 expression by targeting Cbl proto-oncogene B (CBLB) and downregulated MHC-I expression by increasing the level of eukaryotic initiation factor 3B (eIF3B) via the targeting of microphthalmia-associated transcription factor (MITF).

A prediction approach to COVID-19 time series with LSTM integrated attention mechanism and transfer learning.

Background: The prediction of coronavirus disease in 2019 (COVID-19) in broader regions has been widely researched, but for specific areas such as urban areas the predictive models were rarely studied. It may be inaccurate to apply predictive models from a broad region directly to a small area. This paper builds a prediction approach for small size COVID-19 time series in a city.

TMED3 promotes prostate cancer via FOXO1a and FOXO3a phosphorylation.

Background: Transmembrane emp24 trafficking protein 3 (TMED3) is associated with the development of several tumors; however, whether TMED3 regulates the progression of prostate cancer remains unclear.

Materials And Methods: Short hairpin RNA was performed to repress TMED3 in prostate cancer cells (DU145 cells) and in a prostate cancer mice model to determine its function in prostate cancer and .

Results: In the present study, we found that TMED3 was highly expressed in prostate cancer cells.

Paroxetine promotes longevity via ser-7-dop-4-IIS axis in Caenorhabditis elegans.

Paroxetine, a selective serotonin reuptake inhibitor, is widely used in the clinical treatment of depression. While several antidepressants show promise as geroprotectors, the role of paroxetine in aging remains unclear. In this study, we evaluated the lifespan extension effect of paroxetine in Caenorhabditis elegans (C.

Prevalence and Clinical Implications of Pulmonary Vein Stenosis in Bronchiectasis: A 3D Reconstruction CT Study.

Background: Recent studies on bronchiectasis have revealed significant structural abnormalities and pathophysiological changes. However, there is limited research focused on pulmonary venous variability and congenital variation. Through our surgical observations, we noted that coarctation of pulmonary veins and atrophied lung volume are relatively common in bronchiectasis patients.

Rapid Analysis of Nucleic Acids by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry With a Data Algorithm.

Rationale: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a powerful method for identifying viruses via nucleic acid detection. The data processing method is critical in recognizing nucleic acid obtained by MALDI-TOF-MS. Therefore, new development of data algorithm is needed for virus identification.

Dual-Amplification Single-Particle ICP-MS Strategy Based on Strand Displacement Amplification-CRISPR/Cas12a Amplification for Homogeneous Detection of miRNA.

MicroRNAs (miRNAs) regulate a myriad of biological processes and thus have been regarded as useful biomarkers in biomedical research and clinical diagnosis. The specific and highly sensitive detection of miRNAs is of significant importance. Herein, a sensitive and rapid dual-amplification elemental labeling single-particle inductively coupled plasma-mass spectrometry (spICP-MS) analytical method based on strand displacement amplification (SDA) and CRISPR/Cas12a was developed for miRNA-21 detection.