4D-DIA-Based Quantitative Proteomic Analysis Reveals the Involvement of TRPV2 Protein in Duck Tembusu Virus Replication.

Duck Tembusu virus (DTMUV), a novel positive-sense RNA virus, has caused significant economic losses in the poultry industry of Eastern and Southeast Asia since its outbreak in 2010. Furthermore, the rapid transmission and potential zoonotic nature of DTMUV pose a threat to public health safety. In this study, a 4D-DIA quantitative proteomics approach was employed to identify differentially expressed cellular proteins in DTMUV-infected DF-1 cells, which are routinely used for virus isolation and identification for DTMUV, as well as the development of vaccines against other poultry viruses.

Light-Powered Self-Translation of an Asymmetric Friction Slider Using a Liquid Crystal Elastomer String Oscillator.

In recent years, there have been many studies focused on improving the performance of active materials; however, applying these materials to active machines still presents significant challenges. In this study, we introduce a light-powered self-translation system for an asymmetric friction slider using a liquid crystal elastomer (LCE) string oscillator. The self-translation system was composed of a hollow slide, two LCE fibers, and a mass ball.

Folic Acid-Targeted Mixed Pluronic Micelles for Delivery of Triptolide.

The present study aimed to explore an ideal delivery system for triptolide (TPL) by utilizing the thin-film hydration method to prepare drug-loaded, folate-modified mixed pluronic micelles (FA-F-127/F-68-TPL). Scanning electron microscopy and atomic force microscopy showed that the drug-loaded micelles had a spherical shape with a small particle size, with an average of 30.7 nm.

Genome-Wide Identification and Expression Analysis of Gene Family Members in Seashore Paspalum Under Salt Stress.

The gene family plays a crucial role in plant growth, development, and responses to biotic and abiotic stresses. , a warm-season turfgrass with exceptional salt tolerance, can be irrigated with seawater. However, the gene family in seashore paspalum remains poorly understood.

Dietary N-6 Polyunsaturated Fatty Acid Intake and Brain Health in Middle-Aged and Elderly Adults.

Background: Dietary intake of polyunsaturated fatty acids (PUFA) plays a significant role in the onset and progression of neurodegenerative diseases. Since the neuroprotective effects of n-3 PUFA have been widely validated, the role of n-6 PUFA remains debated, with their underlying mechanisms still not fully understood.

Methods: In this study, 169,295 participants from the UK Biobank were included to analyze the associations between dietary n-6 PUFA intake and neurodegenerative diseases using Cox regression models with full adjustments for potential confounders.

Bioactive Secondary Metabolites from Against .

Root-knot nematodes (RKNs) are pathogens that endanger a wide range of crops and cause serious global agricultural losses. In this study, we investigated metabolites of the endoparasitic fungus YMF1.01751, with the expectation of discovering valuable biocontrol compounds.

BLV-CoCoMo Dual qPCR Assay Targeting LTR Region for Quantifying Bovine Leukemia Virus: Comparison with Multiplex Real-Time qPCR Assay Targeting Region.

The proviral load (PVL) of the bovine leukemia virus (BLV) is a useful index for estimating disease progression and transmission risk. Real-time quantitative PCR techniques are widely used for PVL quantification. We previously developed a dual-target detection method, the "Liquid Dual-CoCoMo assay", that uses the coordination of common motif (CoCoMo) degenerate primers.

Diagnostic Accuracy of Antigen-Based Skin Tests (TBSTs) for Tuberculosis Infection Compared with TST and IGRA: A Network Meta-Analysis.

The aim of this study was to evaluate the diagnostic accuracy of the IGRA, TST, and TBST by combining diagnostic test accuracy (DTA) analysis and network meta-analysis (NMA) to increase the reliability and accuracy of diagnostic methods and promote the eradication of TB. An electronic search of the PubMed, Embase, and Cochrane databases was conducted, from the date of establishment to September 30, 2024. Data were synthesized with frequentist random-effects network meta-analyses, a single-group rate meta-analysis algorithm, and a bivariate mixed-effects logistic regression model.

Advancements in the Application of scRNA-Seq in Breast Research: A Review.

Single-cell sequencing technology provides apparent advantages in cell population heterogeneity, allowing individuals to better comprehend tissues and organs. Sequencing technology is currently moving beyond the standard transcriptome to the single-cell level, which is likely to bring new insights into the function of breast cells. In this study, we examine the primary cell types involved in breast development, as well as achievements in the study of scRNA-seq in the microenvironment, stressing the finding of novel cell subsets using single-cell approaches and analyzing the problems and solutions to scRNA-seq.

Indocyanine Blue (ICB) as a Functional Alternative to Indocyanine Green (ICG) for Enhanced 700 nm NIR Imaging.

Despite significant advancements in bioimaging technology, only a limited number of fluorophores are currently approved for clinical applications. Indocyanine green (ICG) is the first FDA-approved near-infrared (NIR) fluorophore and has significantly advanced clinical interventions over the past three decades. However, its single-channel imaging at 800 nm emission is often insufficient for capturing comprehensive diagnostic information during surgery.

Alterations in the Microbiomes and Metabolic Profiles of the Ileal Between the Hu Sheep and East Friesian Sheep.

The East Friesian sheep is a dairy breed known for its high fertility and high milk production and is currently one of the best dairy sheep breeds in the world. This breed is known to have a poor disease-resistant phenotype compared to Hu sheep. Gut microbiota and metabolites play a role in host disease resistance.

Effect of Music Intervention on Improving the Early Prognosis of the Preterm Infant in Chongqing, China: A Randomized Controlled Trial.

Objectives: To investigate the effects of music intervention on the vital signs, weight gain, feeding, hospital stays, and cost of premature infants.

Methods: 100 premature infants were randomized into two groups: the experimental group (given music for 30 min at a time, once every day until discharge) and the control group (without music). To compare the vital signs (RR, HR, SPO) before, during, and after the music intervention, as well as the weight gain and feeding, follow up to 3 months after discharge.

Examining Family Atmosphere and School Climate Within Psychology of Working Theory Among Chinese Rural College Students.

The employment situation for college students has worsened due to the increase in the number of graduates and the impact of the COVID-19 pandemic on the economy. Consequently, the pressure to find employment has also increased, particularly for rural college students. Drawing on the Psychology of Working Theory (PWT), the present study was performed to examine the applicability of some proposed pathways in the PWT and test the relationships between family atmosphere and school climate and work volition, career adaptability, and future decent work perception within 946 rural college students (243 men and 703 women; Mage = 19.

Research Progress in the Extraction, Structural Characteristics, Bioactivity, and Commercial Applications of Oat β-Glucan: A Review.

Oats ( L.) are an important cereal crop with diverse applications in both food and forage. Oat β-glucan has gained attention for its beneficial biological activities, such as reducing cardiovascular risk, preventing diabetes, and enhancing intestinal health.

Research Progress of Liquid Biopsy Based on DNA Methylation in Tumor Diagnosis and Treatment.

Liquid biopsy has been gradually applied to the clinical diagnosis and treatment of tumors because of its non-invasive and real-time reflection of the tumor status, as well as the convenience of sample collection, which allows the detection of primary or metastatic malignant tumors and reflects the heterogeneity of the tumors. DNA methylation, which is a type of epigenetic modification, is essential in the progression of tumors. This review introduces the common DNA methylation analysis methods and discusses their advantages and disadvantages, focusing on the new progress of DNA methylation-based liquid biopsy in tumor diagnosis and treatment.

Mulberry Branch Extracts Enhance the Antioxidant Capacity of Broiler Breast Muscle by Activating the Nrf2 and Cytochrome P450 Signaling Pathway.

Mulberry branch extracts (MBEs) have garnered significant attention as natural feed additives and antioxidants; however, their antioxidant properties in meat post-slaughter and their influence on muscle-related metabolic processes remain largely unexplored. Herein, we evaluated the effects of MBEs on the antioxidant capacity and metabolic processes of breast muscle in yellow-feather broilers by adding 0 g/kg, 1.5 g/kg, 3.

The Bioaccumulation, Fractionation and Health Risk of Rare Earth Elements in Wild Fish of Guangzhou City, China.

In this study, the total content of REEs ranged from 1.32 to 67.74 μg/kg, with a predominant presence of light REEs.

Microbial community analysis of supragingival plaque in patients with fixed prostheses.

Statement Of Problem: Harmony between prostheses and periodontal tissues is essential. The presence of a fixed prosthesis has been reported to increase the risk of periodontal lesion onset in abutment teeth and to affect longevity. However, studies comparing the supragingival plaque biofilm on fixed prostheses and natural teeth are lacking.

Associations between multiple metabolic biomarkers with steatotic liver disease subcategories: A 5-year Chinese cohort study.

The effectiveness of established biomarkers for non-alcoholic fatty liver disease (NAFLD) within the updated framework of steatotic liver disease (SLD) remains uncertain. This cohort study examines the association of four metabolic biomarkers-retinol-binding protein 4 (RBP-4), fibroblast growth factor 21 (FGF-21), adiponectin, and osteocalcin-with SLD and its subtypes: metabolic dysfunction-associated steatotic liver disease (MASLD) and metabolic dysfunction with alcohol-related liver disease (MetALD)/alcohol-related liver disease (ALD). Among 3,504 Chinese participants aged 55-70, 938 (26.

Experimental study on hydrophysical properties and slope planting of ecological composite material solidified loess.

The construction of engineering projects in the Chinese Loess Plateau has resulted in large areas of exposed slopes, increasing the risk of soil erosion. Restoring the slope ecosystem is an effective means to reduce soil erosion, prevent soil and water loss, and maintain slope stability. Ecological slope protection using bio-gum solidified fiber-reinforced loess (GFSL) has been proven to achieve good vegetation restoration effects, but there remains a problem of low vegetation coverage in the early stage of protection.

Bimetallic nanoreactor mediates cascade amplification of oxidative stress for complementary chemodynamic-immunotherapy of tumor.

As a promising tumor treatment, chemodynamic therapy (CDT) can specifically catalyze HO into the cytotoxic hydroxyl radical (·OH) via Fenton/Fenton-like reaction. However, the limited HO and weakly acidic pH in tumor microenvironment (TME) would severely restrict the therapeutic efficiency of CDT. Here, a weakly acid activated, HO self-supplied, hyaluronic acid (HA)-functionalized Ce/Cu bimetallic nanoreactor (CBPNs@HA) is elaborately designed for complementary chemodynamic-immunotherapy.

Dipeptidyl peptidase 4 is a cofactor for porcine epidemic diarrhea virus infection.

Porcine epidemic diarrhea virus (PEDV) is a member of the genus Alphacoronavirus in the family Coronaviridae, which has a mortality rate of up to 100 % in neonatal piglets and causes huge economic losses to the pig industry. The target cells of PEDV infection are porcine small intestinal epithelial cells, and the mechanism of PEDV invasion remains unclear. Our study found that dipeptidyl peptidase 4 (DPP4) acts as a cofactor for PEDV infection by promoting PEDV invasion and replication.

An exploratory study evaluated the 30 most commonly reported medications in the United States food and drug administration's adverse event reporting system that are associated with the occurrence of kidney stones.

Objective: This research project aimed to identify and analyze the top 30 drugs most commonly associated with kidney stone formation using data from the U.S. Food and Drug Administration's Adverse Event Reporting System (FAERS) database.

Lipid bilayers determine the allostery but not intrinsic affinity of cAMP binding to pacemaker channels.

In the human heart, the binding of cyclic adenosine monophosphate (cAMP), a second messenger, to hyperpolarization and cyclic nucleotide-gated (HCN) regulates the automaticity of pacemaker cells. Recent single-molecule binding studies show that cAMP bound to each subunit of purified tetrameric HCN channels independently, in contrast to findings in cells. To explore the lipid membrane's role in cAMP regulation, we reconstituted purified human HCN channels in various lipid nanodiscs and resolved single molecule ligand-binding dynamics.