Renal involvement in Russell's viper bite patients without disseminated intravascular coagulation.

We measured urinary beta-N-acetylglucosaminidase (NAG: EC 3.2.1.

Effects of Russell's viper venom on renal lysosomal functions in experimental mice.

The lysosome-enriched fraction of mice kidney was isolated by homogenization and differential centrifugation. Lysosomal functions, namely lysosomal enzyme activities and membrane integrities were investigated in (a) the renal lysosome-enriched fraction, incubated with different concentrations of Russell's viper venom (RVV) for various time intervals (in vitro test) and (b) the kidney homogenate of mice, which had been envenomed with different dosages of RVV and been sacrificed after various time intervals post-envenomation (in vivo test). Three typical marker enzymes for lysosome were used, namely N-acetyl-beta-D-glucosaminidase (NAG), cathepsin D and acid phosphatase.