In Vitro Activity of Bacteriophages Against Ocular Methicillin-resistant S. aureus Isolates Collected in the US.

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of sight-threatening infections in the US. These strains pose a significant challenge in managing ocular infections, as they frequently exhibit resistance to first-line empirical antibiotics. To assess the potential of bacteriophages as innovative topical therapies for treatment of recalcitrant ocular infections, we evaluated the in vitro antimicrobial activity of a set of anti-S.

Efficacy and safety of larotrectinib in patients with TRK fusion gastrointestinal cancer.

Background: Larotrectinib is the first-in-class, highly selective TRK inhibitor with demonstrated efficacy in various TRK fusion solid tumours. We report the efficacy and safety of larotrectinib in patients with TRK fusion gastrointestinal (GI) cancer.

Methods: Patients with TRK fusion GI cancer from NAVIGATE (NCT02576431) were included.

Theoretical and Experimental Insights into the Chemiresistive Sensing Response of Graphene Quantum Dots: The Role of Oxygen Functional Groups.

Developing sensitive sensors to trimethylamine (TMA) remains a topic of great interest in areas such as food quality analysis and disease biomarkers. To address this issue, chemiresistive sensors were proposed using graphene quantum dots (GQDs) with different proportions of hydroxyl (GQDs-OH), epoxy (GQDs-epoxy), and carboxyl (GQDs-COOH) groups. These materials exhibited different sensitivities to TMA, with GQDs-OH being the most sensitive, presenting a detection limit of 0.

Construction of a Simplex Search Framework for the Development of a New Specific and Robust Stability-Indicating Reversed-Phase High-Performance Liquid Chromatography Assay: Application for the Stability of a Peptide-Based Telomerase Vaccine.

In this work, a novel mathematical algorithm was described for the development of a high-performance liquid chromatography (HPLC) stability-indicating assay. This approach was applied for the stability study of a universal cancer peptide-based telomerase vaccine constituted of a mixture of two peptides. With only 18 experiments, the optimum HPLC gradient conditions were determined using a computer-assisted design of experiments-simplex algorithm software.

Feeder-cell-free system for production of natural killer cells from cord blood hematopoietic stem and progenitor cells.

Introduction: Natural Killer (NK) cells hold significant promise as therapeutic agents in immuno-oncology due to their ability to target and eliminate cancerous and infected cells without causing graft-versus-host disease or cytokine release syndrome. However, the limited availability of robust, scalable methods for generating clinical-grade NK cells remains a limiting factor to broader clinical application.

Methods: Here we report the development of a novel feeder-cell-free culture system optimized for producing NK cells from cord blood-derived CD34 hematopoietic stem and progenitor cells (HSPCs).

First molecular detection of Bartonella spp. in two chewing louse species (Amblycera: Menoponidae and Ischnocera: Philopteridae) parasitizing Calonectris borealis (Cory, 1881) (Procellariiformes: Procellariidae).

Procellariidae is the most diverse family of the order Procellariiformes, with 13 genera and about 90 species of seabirds. The genus Calonectris is represented by three species in the Atlantic Ocean. Bartonella spp.

Force and Gait Mechanics at Reduced Body Weight on a Lower Body Positive Pressure Treadmill.

Lower body positive pressure treadmills (LBPPT) have gained increasing attention due to their potential applications in sports training, rehabilitation, and biomechanics research. However, there is limited understanding of how differing body weight percentages influence gait and force production. This study aimed to investigate the relationship between force and gait parameters at different body weight percentages on a LBPPT.

A Comparison of Radiography, X-Ray Tomosynthesis, and CT for Intraorbital Metallic Foreign Body Screening.

Objective: Before MRI examination, patients are often screened for intraorbital metallic foreign bodies (IMFBs) via questioning, clinical record review, and imaging when appropriate. This work compares the screening performance of digital radiography (DR), digital tomosynthesis (DT), and CT in detecting IMFBs composed of various metals and identifies their limits of detection.

Methods: Fragments of aluminum, steel, and cobalt-cemented tungsten carbide were produced.

Impact of tumor and node stages on the efficacy of adjuvant oxaliplatin-based chemotherapy in stage III colon cancer patients: an ACCENT pooled analysis.

Background: Standard adjuvant treatment of stage III colon cancer (CC) is fluoropyrimidine with oxaliplatin. Recently, stage III was subdivided into low-risk (T1-3, N1) and high-risk (T4 and/or N2), with the benefit of adding oxaliplatin varying across these substages. In this study, we aimed to assess the impact of oxaliplatin on survival outcomes in subdividing stage III CC patients based on T and N staging.

Antisense-mediated regulation of exon usage in the elastic spring region of Titin modulates sarcomere function.

Background: Alternative splicing of Titin (TTN) I-band exons produce protein isoforms with variable size and elasticity, but the mechanisms whereby TTN splice factors regulate exon usage and thereby determining cardiomyocyte passive stiffness and diastolic function, is not well understood. Non-coding RNA transcripts from the antisense strand of protein-coding genes have been shown to regulate alternative splicing of the sense gene. The TTN gene locus harbours >80 natural antisense transcripts (NATs) with unknown function in the human heart.

A Screw Length of 14 mm Is Sufficient to Avoid the Physis During Juvenile Osteochondritis Dissecans Lesion Repair but This Can Vary Based on Age and Region of the Distal Femur.

Purpose: To determine the safest screw length that will avoid penetrating the femoral physis for surgical fixation of juvenile osteochondritis dissecans lesions and to compare the safe screw lengths for different regions in the condyles.

Methods: After institutional review board approval, we retrospectively reviewed T1-weighted magnetic resonance images of pediatric patients. Longitudinal axis reference lines were made on the femur.

Characterisation of Polymer Materials for the Development of an Artificial Urethra.

Low Urinary Tract Dysfunctions (LUTD) and in particular Urinary Incontinence (UI) is a medical condition affecting millions of people worldwide. In this context researchers and engineers performed tests on porcine and human urethras. However, ethical concerns and limited human tissue availability make direct testing on human urethras complicated.

Hemogregarines in bufonid anurans from the Brazilian Amazon, with description a new species of (Apicomplexa: Lankesterellidae).

Here we describe, in an unprecedented way for the Brazilian Amazon, one Apicomplexa species infecting an anuran in Pará, Brazil, using an integrative approach that includes taxonomy, providing morphology, morphometrics, and molecular data. Samples were collected between February 2021 and February 2022 in the community of Curupira, municipality of Santarém, and in the industrial area of Marabá, both in Pará state. Specimens of and were captured by hand during the active search, and blood samples were collected by cardiac puncture.

Prediction of relapse in a French cohort of outpatients with schizophrenia (FACE-SZ): Prediction, not association.

Background: Schizophrenia (SZ) commonly manifests through multiple relapses, each impeding the path to recovery and incurring personal and societal costs. Despite the identification of various risk factors associated to the risk of relapse, the development of accurate algorithms predictive of relapse has been limited, partly due to inadequate statistical methods. Additionally, despite the wealth of data showing strong associations between inflammation and schizophrenia, the two existing studies failed to demonstrate whether inflammatory parameters could predict relapse.

A new module in the drug development process: preclinical multi-center randomized controlled trial of R-ketamine on alcohol relapse.

The drug development process in psychiatry faces significant challenges due to low reproducibility rates in animal testing, which often leads to translation failures. To address this issue, we introduce a new approach in psychiatric drug development: a preclinical randomized controlled trial (preRCT). To demonstrate its potential utility, we conducted a multi-center preRCT using the alcohol deprivation effect (ADE) model to assess the impact of ketamine and R-ketamine on alcohol relapse across three European research centers.

Conversion during Minimally Invasive Left Pancreatectomy: A Nationwide Study of Causes and Consequences.

Objectives: To identify risk factors for conversion, develop a predictive Conversion Risk Score (CRS), and assess the association between conversion and severe postoperative complications.

Background: Conversion occurs in 15-30% of minimally invasive left pancreatectomies (MILP). Risk factors and potential negative impacts on postoperative outcomes are poorly described.

Deep Learning-based Aligned Strain from Cine Cardiac MRI for Detection of Fibrotic Myocardial Tissue in Patients with Duchenne Muscular Dystrophy.

Purpose To develop a deep learning (DL) model that derives aligned strain values from cine (noncontrast) cardiac MRI and evaluate performance of these values to predict myocardial fibrosis in patients with Duchenne muscular dystrophy (DMD). Materials and Methods This retrospective study included 139 male patients with DMD who underwent cardiac MRI examinations at a single center between February 2018 and April 2023. A DL pipeline was developed to detect five key frames throughout the cardiac cycle, and respective dense deformation fields, allowing for phase-specific strain analysis across patients and from one key frame to the next.

Diversity of spp. (Piroplasmida: Theileriidae) in Wild Felids from Brazil and Argentina.

Domestic and wild felids are frequently parasitized by apicomplexan protozoa in the genus . Expanding species diversity has recently been described within this genus, with potential implications for epidemiology and pathogenesis. In light of these findings, this study assessed the genetic diversity of spp.

Enhancing Patient Safety Education: Cross-Cultural Validation of the APSQ-III in Brazilian Healthcare Students.

This study aimed to adapt and validate the Attitudes to Patient Safety Questionnaire (APSQ-III) for Brazilian Portuguese and to compare patient safety attitudes between medical and nursing students. Given the critical role of assessing safety attitudes in shaping future healthcare professionals, this research addresses a significant gap in the Brazilian educational context. The cross-cultural adaptation process adhered to the guidelines of for the Process of Cross-Cultural Adaptation of Self-Report Measures, encompassing translation, synthesis, back-translation, and expert committee evaluation.

Immune Alterations and Viral Reservoir Atlas in SIV-Infected Chinese Rhesus Macaques.

Background/objectives: Over the last decades, our projects have been dedicated to clarifying immunopathological and virological events associated with Human Immunodeficiency Virus (HIV) infection.

Methods: By using non-human primate models of pathogenic and non-pathogenic lentiviral infections, we aimed at identifying the cells and tissues in which the virus persists, despite antiretroviral therapy (ART). Indeed, the eradication of viral reservoirs is a major challenge for HIV cure.

Unveiling protective mechanisms of wild olive (acebuche) oil in retinal pigment epithelial cells with hypertensive phenotype.

Arterial hypertension leads to oxidative and inflammatory imbalances, triggering hypertensive organ damage through several pathways. We have previously described the antioxidant and anti-inflammatory properties of olive oil extracted from the wild olive tree (Olea europaea var. sylvestris, acebuche, ACE) against hypertensive ocular damage.

Strengthening effect of flavonoid antioxidant on resin-enamel bond strength following tooth bleaching.

This study aimed to assess the impact of flavonoid-based antioxidant application after in-office bleaching with 35% hydrogen peroxide on the shear bond strength of resin to bovine enamel, comparing it with 10% sodium ascorbate. Bovine enamel blocks (n = 10) were randomly assigned to one of five pretreatments: (i) no bleaching, (ii) bleaching without antioxidant application, and (iii) bleaching followed by a 1-min application of 10% sodium ascorbate, (iv) 5% naringin, or (v) 10% naringin. Color analysis was conducted using a digital spectrophotometer.

Ischemic stroke care for patients affiliated to the French agricultural health insurance scheme: A national study.

Background: Health disparities along the rural-urban spectrum are a growing concern. The objective of this study was to determine whether in France, clinical profile, care and outcome after ischemic stroke differed between patients affiliated to the statutory health insurance system dedicated to the agricultural economic sector (i.e.

Novel Sulfonamide-Sydnone Hybrids: Complementary Insight into Anti-Inflammatory Action, Anti-SARS-CoV-2 Activity, Human Serum Albumin Interaction, and in silico Analysis.

Acute lung injury (ALI) is a severe condition often seen in intensive care unit patients. Due to limited treatment options, ALI is linked to high rates of mortality and morbidity. Bacterial and viral infections are significant contributors to ALI.

[Psychiatry and life-threatening emergencies, training for user safety].

At the Loire-Atlantique Nord psychiatric facility, which is located in a rural area, responding to life-threatening emergencies can be complex due to a lack of practice and delays in emergency response. In 2020, collaboration with care management and nurses sensitive to this issue identified a need for improvement. Emergency care training was introduced in the form of workshops to enhance caregivers' skills and guarantee patient safety.