Bioavailability of tryptophan in soybean meal for 10-kg pigs using slope-ratio assay.

A basal diet formulated to contain 1.1 (analyzed to contain 1.03) g of tryptophan/kg diet as-fed, but adequate in all other amino acids, was supplemented with .

Phytase-induced changes in mineral utilization in zinc-supplemented diets for pigs.

Forty-eight pigs (barrows:gilts, 1:1) with an average initial weight of 9.4 kg were used in a 2 x 2 factorial experiment to determine the influence of dietary phytase (0 or 1,500 phytase units/kg) and zinc (0 or 100 mg/kg) supplementation of a corn-soybean meal diet on the utilization of P, Ca, Cu, Mg, Mn, and Zn. After a 21-d growth experiment, feed was withheld for 24 h and blood was collected from the anterior vena cava of all pigs for plasma mineral analyses.

Isolation and photodynamic effects of hematoporphyrin derivative components: a chromatographic analysis of the starting materials.

Twenty different fractions of hematoporphyrin derivatives (HpD) and eight fractions of an HpD dimer mixture were isolated utilizing isocratic reversed-phase ion-pair high-performance liquid chromatography. These fractions were characterized by UV-visible and fluorescence spectrophotometry. Fluorescence quantum yields and photokill efficiency for each fraction in PTK2 epithelial cells were obtained.

Nutrient digestibility and growth performance of pigs fed pearl millet as a replacement for corn.

Three experiments were conducted to determine the value of pearl millet grain, grown at either Purdue University or the University of Nebraska, as a substitute for corn in the diets of young pigs. In Exp. 1, 24 crossbred barrows, average initial weight of 20.

Nitrogen utilization and lean growth performance of 20- to 50-kilogram pigs fed diets balanced for lysine:energy ratio.

Two experiments were conducted to determine the lysine:DE ratio that would maximize nitrogen retention and lean growth in growing pigs. Diets were formulated to contain 2.5, 3.

Availability of amino acids for 10- to 20-kilogram pigs: lysine and threonine in soybean meal.

The availabilities of lysine and threonine in soybean meal for 10- to 20-kg pigs were determined in two experiments using slope-ratio methodology. In the first experiment, a basal diet formulated to contain 6 g of lysine/kg, but adequate in all other amino acids, was supplemented with 1, 2, or 3 g of lysine/kg from either soybean meal or L-lysine-HCl. Each of the seven diets was available on an ad libitum basis to six individually penned pigs (barrows:gilts, 1:1) from an initial weight of 9.

Pearl millet in diets of white Pekin ducks.

In two 21-d experiments, the performance, nutrient utilization, and carcass composition of ducks fed diets containing pearl millet were compared with those fed diets containing corn. Corn, in diets at two protein levels (22 vs 18% in Experiment 1 and 22 vs 16% in Experiment 2), was replaced by pearl millet either on an equal-weight or isonitrogenous basis. During the first 2 wk of the first experiment, corn diets compared on an isonitrogenous basis were superior (P < .

Comparative extraction methods for spectrophotometric analysis of uric acid in avian excreta.

Different extraction techniques were compared in uric acid analysis of avian excreta. These include five extraction reagents (0.1 M sodium hydroxide, 0.

Ameloblastoma: clinical features and management of 315 cases from Kaduna, Nigeria.

This paper reviews 315 cases of ameloblastoma seen and managed at the Oral and Maxillofacial Clinic of Ahmadu Bello University Hospital, Kaduna, Nigeria over a 20-year period. The data collected on age at presentation, sex distribution, clinical presentation and modalities of treatment are analysed and discussed. A male preponderance was found, with peak presentation in the third and fourth decades of life.

Regional brain neurotransmitter concentrations in stress-susceptible pigs.

In three experiments, the concentrations of indoleamines and catecholamines were measured in the brain regions of pigs that were homozygous-positive (nn, stress-susceptible), heterozygous (Nn), and homozygous-negative (NN, stress-tolerant) for the halothane gene. In Exp. 1 (n = 10), hypothalamic 5-hydroxytryptophan (P < .

Evaluation of long-term somatotropin implants in finishing pigs.

Ninety pigs were used to investigate the efficacy of long-term implants of porcine somatotropin (pST) in improving growth, feed:gain ratio, and carcass characteristics in pigs at three dose levels (0-, 100-, and 200-mg pST implant) from 71 to 99 kg live weight. The use of long-term implants did not alter (P > .05) growth rate.

Metabolic responses induced by isoproterenol in ractopamine-fed pigs.

Ractopamine at 0 or 20 mg/kg and protein at 130 or 170 g/kg diet were used in a 2 x 2 factorial experiment arranged in six randomized complete blocks of 64-kg pigs. On d 21, pigs were intravenously administered 1 microgram isoproterenol/kg body wt, and blood was sampled at -60, -30, 0, 5, 10, 15, 20, 30, 60, 90 and 120 min relative to injection. Subcutaneous adipose tissue samples were used to measure in vitro lipolytic and lipogenic responses to isoproterenol.

Hypothalamic neurotransmitter concentrations and meat quality in stressed pigs offered excess dietary tryptophan and tyrosine.

Pale, soft, exudative (PSE) pork occurs, for the most part, from environmental stress on the pig. Amino acid intake may be related to stress susceptibility through hormone and neurotransmitter induction. Two experiments were conducted to determine whether supplementation of 5 g of tryptophan (TRP) or 10 g of tyrosine (TYR) per kilogram of a 14% CP diet would alter the response of pigs to stress as measured by hypothalamic neurotransmitter concentrations and incidence of PSE.

Porcine skeletal muscle myofibrillar protein synthesis is stimulated by ractopamine.

Thirty-two 64-kg individually penned barrows were fed protein at 130 or 170 g/kg diet and ractopamine at 0 or 20 mg/kg in a randomized complete block design for 28 d. Fractional synthesis rates of myofibrillar, sarcoplasmic and connective tissue proteins in longissimus dorsi, biceps femoris and gastrocnemius muscles were examined by primed-continuous infusion of L-[ring-2,6-3H(N)]phenylalanine over a 4-h period. Fractional synthesis, accretion and breakdown rates of protein in the muscles were not affected by dietary ractopamine, regardless of whether estimated on the basis of plasma or tissue homogenate specific activity.

Manipulation of porcine carcass composition by ractopamine.

The effect of dietary ractopamine and protein level on growth performance, individual muscle weight and carcass composition of finishing pigs were evaluated in two experiments. Twelve barrows and 12 gilts (Exp. 1) and 32 barrows (Exp.

In vitro Na+,K+-ATPase (EC 3.6.1.3)-dependent respiration and protein synthesis in skeletal muscle of pigs fed at three dietary protein levels.

1. Eighteen pigs were offered diets containing 130, 170 or 210 g protein/kg with three barrows and three gifts per diet from 20 to 60 kg live weight. Oxygen consumption, Na+,K+-ATPase (EC 3.

Dietary protein-induced changes in porcine muscle respiration, protein synthesis and adipose tissue metabolism.

Growth rate, physically separable tissues of the ham and loin, heat production, skeletal muscle respiration and protein synthesis, and lipogenesis and lipolysis in s.c. adipose tissue were measured in a single experiment in which pigs were offered a 13 (n = 8), or 21% (n = 6) protein diet from 20 to 100 kg live weight.