Effect of hypoxia on HIF-1α and NOS3 expressions in CD34 cells of JAK2V617F-positive myeloproliferative neoplasms.

Purpose: Myeloproliferative neoplasms (MPN) are a heterogeneous group of hematopoietic stem-cell diseases with excessive proliferation of one or more blood cell lines. In this study, we evaluated the effect of different oxygen concentrations on HIF-1α and NOS3 gene expression to determine the effect of the bone marrow microenvironment on JAK2V617F positive Philadelphia chromosome negative (Ph) MPNs.

Patients And Methods: Peripheral blood mononuclear cells (MNC) of 12 patients with Ph MPN were collected.

Circulating CD133+/–CD34– Have Increased c- Expression in Myeloproliferative Neoplasms.

Objective: Myeloproliferative neoplasms (MPNs) are hematopoietic stem cell (HSC)-originated diseases with clonal myeloproliferation. The constitutive activation of the JAK/STAT pathway is frequently detected in patients with Philadelphia chromosome-negative (Ph–) MPNs with an acquired V617F mutation. The c- proto-oncogene is associated with malignant growth and cellular transformation, and V617F was previously shown to induce constitutive expression of c-.